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Induction of the Nrf2-driven antioxidant response by tert-butylhydroquinone prevents ethanol-induced apoptosis in cranial neural crest cells
- Source :
- Biochemical pharmacology. 80(1)
- Publication Year :
- 2010
-
Abstract
- Previous studies have shown that ethanol exposure causes apoptosis in cranial neural crest cells (NCCs), an ethanol-sensitive cell population implicated in Fetal Alcohol Spectrum Disorders (FASD). Additionally, induction of endogenous antioxidants through activation of nuclear factor-erythroid 2-related factor 2 (Nrf2) has been shown to prevent oxidative stress and apoptosis in ethanol-exposed mouse embryos. The objective of this study was to test whether tert-butylhydroquinone (tBHQ), an Nrf2 inducer, can protect NCCs against ethanol-induced apoptosis. Ethanol exposure was shown to cause a moderate increase in the protein expression of Nrf2 and its downstream antioxidants in the NCCs. Treatment of NCCs with tBHQ alone significantly increased the protein expression of Nrf2 and its downstream antioxidants and also significantly increased the activities of the antioxidant enzymes. In NCCs exposed to ethanol, the tBHQ-mediated antioxidant response prevented oxidative stress and apoptosis. These results clearly demonstrate that the activation of Nrf2 signaling confers protection against ethanol-induced apoptosis in NCCs.
- Subjects :
- tert-Butylhydroquinone
Antioxidant
NF-E2-Related Factor 2
medicine.medical_treatment
Population
Apoptosis
Biology
medicine.disease_cause
Biochemistry
Antioxidants
Article
chemistry.chemical_compound
Mice
Cranial neural crest
Pregnancy
medicine
Animals
Inducer
education
Cells, Cultured
Pharmacology
Neurons
education.field_of_study
Ethanol
Neural crest
Embryo, Mammalian
Cell biology
Hydroquinones
Mice, Inbred C57BL
Oxidative Stress
chemistry
Neural Crest
Female
Oxidative stress
Signal Transduction
Subjects
Details
- ISSN :
- 18732968
- Volume :
- 80
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Biochemical pharmacology
- Accession number :
- edsair.doi.dedup.....8428305b36d54357e0130fae4adc031f