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MiR-129-5p alleviates myocardial injury by targeting suppressor of cytokine signaling 2 after ischemia/reperfusion
- Source :
- Kaohsiung Journal of Medical Sciences, Vol 36, Iss 8, Pp 599-606 (2020)
- Publication Year :
- 2019
-
Abstract
- Acute myocardial infarction (AMI) remains one of the leading causes of morbidity and mortality worldwide. Ischemia/reperfusion (I/R), the most common consequence of AMI treatment, may induce severe myocardial cell injury, yet the precise mechanism continues to be enigmatic. In the present study, we found that miR‐129‐5p was significantly downregulated in mouse I/R model. Then, we overexpressed miR‐129‐5p via intravenous injection of specific miR‐129‐5p agomir before I/R model establishment. MiR‐129‐5p overexpression dramatically alleviated myocardial injury in I/R mice as evidenced by reduced lactate dehydrogenase (LDH) activity, malondialdehyde (MDA) content, and infract size. We further detected the effect of miR‐129‐5p on hypoxia/reoxygenation (H/R)‐induced H9C2 cell in vitro. MiR‐129‐5p overexpression improved H9C2 cell viability and inhibited cell apoptosis induced by H/R, accompanied with decreased LDH activity and MDA content. Besides, luciferase reporter assay indicated that suppressor of cytokine signaling 2 (SOCS2) was a downstream target of miR‐129‐5p. SOCS2 was upregulated in H/R induced H9C2 cells, and miR‐129‐5p overexpression suppressed SOCS2 expression at both mRNA and protein levels. In addition, SOCS2 overexpression abolished the protective effects of miR‐129‐5p on H/R‐induced H9C2 cells, concomitant with elevated expression of apoptosis‐related cleaved poly‐(ADP‐ribose) polymerase and cleaved caspase‐3. In conclusion, our results demonstrated that miR‐129‐5p alleviated myocardial injury induced by I/R both in vitro and in vivo, and miR‐129‐5p/SOCS2 axis is a potential therapeutic target for alleviating myocardial injury in AMI patients after reperfusion.
- Subjects :
- medicine.medical_treatment
Ischemia
Down-Regulation
Myocardial Reperfusion Injury
Suppressor of Cytokine Signaling Proteins
Cell Line
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Downregulation and upregulation
In vivo
Lactate dehydrogenase
medicine
Animals
myocardial injury
SOCS2
lcsh:R5-920
Base Sequence
business.industry
Myocardium
General Medicine
Hypoxia (medical)
medicine.disease
ischemia/reperfusion
Rats
Mice, Inbred C57BL
Disease Models, Animal
MicroRNAs
Cytokine
chemistry
Apoptosis
030220 oncology & carcinogenesis
Cancer research
miR‐129‐5p
030211 gastroenterology & hepatology
medicine.symptom
business
lcsh:Medicine (General)
Subjects
Details
- ISSN :
- 24108650
- Volume :
- 36
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- The Kaohsiung journal of medical sciencesREFERENCES
- Accession number :
- edsair.doi.dedup.....843ec10b363d78ff84d4976ca8d794b5