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Autophagy Regulates Craniofacial Bone Acquisition
- Source :
- Calcif Tissue Int
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Increasing evidence has demonstrated the important role of autophagy in skeletal homeostasis; however, the role of autophagy in craniofacial bone development and acquisition is largely unknown. In this study, we investigated the effect of autophagy suppression on craniofacial bone acquisition by deleting Fip200 or Atg5, two essential autophagy genes, using Osterix-Cre (Osx-Cre). We found that the Osx-Cre transgene mildly decreased the bone mass of parietal bone but not frontal bone, and did not affect cranial base bone mass in adult mice. In the cranial vault, Fip200 or Atg5 deletion similarly decreased 50% bone mass of neural crest-derived frontal bone; Atg5 deletion decreased 50% and Fip200 deletion decreased 30% bone mass of mesoderm-derived parietal bone. In the cranial base, Fip200 or Atg5 deletion similarly decreased 30% bone mass of neural crest-derived presphenoid bone; Atg5 deletion decreased 30% and Fip200 deletion decreased 16% bone mass of mesoderm-derive basioccipital bone. Lastly, we used doxycycline treatment to inhibit the Osx-Cre expression until two months of age and showed that postnatal Fip200 deletion led to cranial vault bone mass decrease in association with a small increase in both bone volume/tissue volume and tissue mineral density. Altogether, this study demonstrated the important role of autophagy in craniofacial bone acquisition during development and postnatal growth.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Endocrinology, Diabetes and Metabolism
ATG5
Mice, Transgenic
030209 endocrinology & metabolism
Calvaria
Biology
Facial Bones
Article
Mice
03 medical and health sciences
0302 clinical medicine
Endocrinology
Internal medicine
Cranial vault
Autophagy
medicine
Animals
Orthopedics and Sports Medicine
Bone Development
Skull
Frontal bone
medicine.anatomical_structure
030101 anatomy & morphology
Parietal bone
Homeostasis
Subjects
Details
- ISSN :
- 14320827 and 0171967X
- Volume :
- 105
- Database :
- OpenAIRE
- Journal :
- Calcified Tissue International
- Accession number :
- edsair.doi.dedup.....844ef9a42ad4bffabefaf2cd61926493