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Ganoderma atrum polysaccharide ameliorates anoxia/reoxygenation-mediated oxidative stress and apoptosis in human umbilical vein endothelial cells
Ganoderma atrum polysaccharide ameliorates anoxia/reoxygenation-mediated oxidative stress and apoptosis in human umbilical vein endothelial cells
- Source :
- International Journal of Biological Macromolecules. 98:398-406
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Ganoderma atrum polysaccharide (PSG-1), a main polysaccharide from Ganoderma atrum, possesses potent antioxidant capacity and cardiovascular benefits. The aim of this study was to investigate the role of PSG-1 in oxidative stress and apoptosis in human umbilical vein endothelial cells (HUVECs) under anoxia/reoxygenation (A/R) injury conditions. The results showed that exposure of HUVECs to A/R triggered cell death and apoptosis. Administration of PSG-1 significantly inhibited A/R-induced cell death and apoptosis in HUVECs. PSG-1-reduced A/R injury was mediated via mitochondrial apoptotic pathway, as evidenced by elevation of mitochondrial Bcl-2 protein and mitochondrial membrane potential, and attenuation of Bax translocation, cytochrome c release and caspases activation. Furthermore, PSG-1 enhanced the activities of superoxide dismutase, catalase and glutathione peroxidase and glutathione content, and concomitantly attenuated reactive oxygen species generation, lipid peroxidation and glutathione disulfide content. The antioxidant, N-acetyl-l-cysteine, significantly ameliorated all of these endothelial injuries caused by A/R, suggesting that antioxidant activities might play a key role in PSG-1-induced endothelial protection. Taken together, these findings suggested that PSG-1 could be as a promising adjuvant against endothelial dysfunction through ameliorating oxidative stress and apoptosis.
- Subjects :
- 0301 basic medicine
Programmed cell death
Cell Survival
Apoptosis
Pharmacology
Biology
medicine.disease_cause
Biochemistry
Antioxidants
Lipid peroxidation
Superoxide dismutase
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Structural Biology
Human Umbilical Vein Endothelial Cells
medicine
Humans
Molecular Biology
bcl-2-Associated X Protein
Membrane Potential, Mitochondrial
chemistry.chemical_classification
Glutathione peroxidase
Cytochromes c
Fungal Polysaccharides
Ganoderma
General Medicine
Glutathione
Oxygen
Oxidative Stress
030104 developmental biology
Proto-Oncogene Proteins c-bcl-2
chemistry
Caspases
030220 oncology & carcinogenesis
biology.protein
Glutathione disulfide
Lipid Peroxidation
Reactive Oxygen Species
Oxidative stress
Subjects
Details
- ISSN :
- 01418130
- Volume :
- 98
- Database :
- OpenAIRE
- Journal :
- International Journal of Biological Macromolecules
- Accession number :
- edsair.doi.dedup.....8489d572abac02ca47c34d0a53c25cb4
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2017.01.071