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Establishment of a gene function analysis system for the euhalophyte Salicornia europaea L
- Source :
- Plant cell reports. 36(8)
- Publication Year :
- 2017
-
Abstract
- A Salicornia europaea L. in vitro cell transformation system was developed and further applied to SeNHX1 function investigation. The exploration of salt-tolerant genes from halophyte has seriously been limited by the lack of self-dependent transformation system. Here, an Agrobacterium tumefaciens-mediated in vitro cell transformation system of euhalophyte Salicornia europaea L. was developed. Calli derived from hypocotyl of S. europaea were co-cultured for 3 days with Agrobacterium at OD600 ranging from 1.0 to 1.5 and then selected with 25 mg/L hygromycin (Hyg). The transformed cells were identified from Hyg positive calli by GUS assay and qRT-PCR, and the transformation efficiency was up to 74.4%. The practicality of this system was further tested via genetic manipulation of S. europaea Na+/H+ antiporter 1 (SeNHX1) gene by creating the overexpressing, silencing, and empty vector cells. Survival ratio and Na+ distribution under salt treatment showed obvious differences in SeNHX1-overexpressing, -silencing, and empty vector cells, indicating the feasibility of this system to analyze gene function. This investigation is enlightening for studies in other non-model plants lacking of self-dependent transformation system.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Salicornia europaea
Sodium-Hydrogen Exchangers
Agrobacterium
Antiporter
GUS reporter system
Plant Science
Chenopodiaceae
Sodium Chloride
01 natural sciences
Hypocotyl
03 medical and health sciences
Transformation, Genetic
Botany
Plant Proteins
biology
fungi
Salt-Tolerant Plants
General Medicine
Agrobacterium tumefaciens
Salt Tolerance
biology.organism_classification
Plants, Genetically Modified
Molecular biology
Transformation (genetics)
030104 developmental biology
Agronomy and Crop Science
010606 plant biology & botany
Transformation efficiency
Subjects
Details
- ISSN :
- 1432203X
- Volume :
- 36
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Plant cell reports
- Accession number :
- edsair.doi.dedup.....84982b45b26011cb0a488fb84abb0765