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Development of a DNA Barcoding-Like Approach to Detect Mustard Allergens in Wheat Flours
- Source :
- Genes, Volume 10, Issue 3, Genes, Vol 10, Iss 3, p 234 (2019)
- Publication Year :
- 2019
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2019.
-
Abstract
- The spread of food allergens is a topic of global importance due to its impact on public health. National and International regulations ask food producers and manufacturers to declare product compositions on the label, especially in case of processed raw materials. Wheat flour (Triticum aestivum) can be contaminated by a wide range of species belonging to the Brassicaceae in the field or during grain harvests, storage, and processing. Among them, mustards (Brassica nigra, Brassica juncea and Sinapis alba) are well known allergenic species. Often, food quality laboratories adopt an ELISA approach to detect the presence of mustard species. However, this approach shows cross-reactivity with other non-allergenic species such as Brassica napus (rapeseed). In the last few years, DNA barcoding was proposed as a valid identification method, and it is now commonly used in the authentication of food products. This study aims to set up an easy and rapid DNA-based tool to detect mustard allergenic species. DNA barcoding (matK and ITS2) and chromosome markers (A6, B, C1 genome regions) were selected, and specific primers were validated on incurred reference food matrices. The developed test was proven to be able to distinguish mustard from rapeseed and wheat, overcoming cross-reactivity with Brassica napus.
- Subjects :
- Rapeseed
allergen detection
lcsh:QH426-470
Sinapis
Flour
Wheat flour
Brassica
Triticum aestivum
Food Contamination
01 natural sciences
DNA barcoding
Article
processed food
0404 agricultural biotechnology
Genetics
DNA Barcoding, Taxonomic
Genetics (clinical)
Triticum
Plant Proteins
biology
business.industry
Allergen
010401 analytical chemistry
Brassica napus
Plant Protein
food and beverages
Brassicaceae
04 agricultural and veterinary sciences
Allergens
biology.organism_classification
040401 food science
0104 chemical sciences
Biotechnology
Mustard Plant
lcsh:Genetics
Food processing
business
Edible Grain
Brassica napu
Subjects
Details
- Language :
- English
- ISSN :
- 20734425
- Database :
- OpenAIRE
- Journal :
- Genes
- Accession number :
- edsair.doi.dedup.....84bbf705d14291510c0a8e63f65aac93
- Full Text :
- https://doi.org/10.3390/genes10030234