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Efficient electrospray ionization from polymer microchannels using integrated hydrophobic membranes
- Source :
- Lab on a chip. 4(4)
- Publication Year :
- 2004
-
Abstract
- A simple process for realizing stable and reliable electrospray ionization (ESI) tips in polymer microfluidic systems is described. The process is based on the addition of a thin hydrophobic membrane at the microchannel exit to constrain lateral dispersion of the Taylor cone formed during ESI. Using this approach, ESI chips are shown to exhibit well-defined Taylor cones at flow rates as low as 80 nL min(-1) through optical imaging. Furthermore, stable electrospray current has been measured for flow rates as low as 10 nL min(-1) over several hours of continuous operation. Characterization of the electrospray process by optical and electrical monitoring of fabricated ESI chips is reported, together with mass spectrometry validation using myoglobin as a model protein. The novel process offers the potential for low-cost, direct interfacing of disposable polymer microfluidic separation platforms to mass spectrometry.
- Subjects :
- chemistry.chemical_classification
Electrospray
Spectrometry, Mass, Electrospray Ionization
Microchannel
Myoglobin
Polymers
Electrospray ionization
Microfluidics
Biomedical Engineering
Analytical chemistry
Membrane Proteins
Bioengineering
General Chemistry
Polymer
Mass spectrometry
Biochemistry
Taylor cone
Membrane
chemistry
Subjects
Details
- ISSN :
- 14730197
- Volume :
- 4
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Lab on a chip
- Accession number :
- edsair.doi.dedup.....84d9f91f669d5858c6ba51c688da8b3b