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[Untitled]
- Source :
- Archives of Virology
-
Abstract
- A closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) assay was developed for the detection of yam mosaic virus (YMV, genus Potyvirus) infecting yam (Dioscorea spp.). The assay uses a set of six oligonucleotide primers targeting the YMV coat protein region, and the amplification products in YMV-positive samples are visualized by chromogenic detection with SYBR Green I dye. The CT-RT-LAMP assay detected YMV in leaf and tuber tissues of infected plants. The assay is 100 times more sensitive in detecting YMV than standard RT-PCR, while maintaining the same specificity. Electronic supplementary material The online version of this article (10.1007/s00705-018-3706-0) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
S1
Potyvirus
Loop-mediated isothermal amplification
Gene Expression
Diamines
Biology
Sensitivity and Specificity
03 medical and health sciences
chemistry.chemical_compound
Virology
Benzothiazoles
Organic Chemicals
Reverse Transcription Loop-mediated Isothermal Amplification
DNA Primers
Fluorescent Dyes
Plant Diseases
Yam mosaic virus
Dioscorea
Chromogenic
Brief Report
Reverse Transcription
General Medicine
biology.organism_classification
Reverse transcriptase
Plant Leaves
Plant Tubers
030104 developmental biology
chemistry
Quinolines
SYBR Green I
Capsid Proteins
Nucleic Acid Amplification Techniques
Subjects
Details
- ISSN :
- 03048608
- Database :
- OpenAIRE
- Journal :
- Archives of Virology
- Accession number :
- edsair.doi.dedup.....84f9636f42bc4c6d9c74e14a0b9f67be