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Real time /spl beta/-imaging with silicon hybrid pixel detectors: kinetic measurements with C-14 amino acids and P-32 nucleotides
- Source :
- IEEE Transactions on Nuclear Science. 49:2213-2217
- Publication Year :
- 2002
- Publisher :
- Institute of Electrical and Electronics Engineers (IEEE), 2002.
-
Abstract
- We present some additional results obtained with the BETAview system, a digital system for real time /spl beta/-imaging based on semiconductor pixel detectors and on a single photon counting read-out chip. We describe the system assembled with a Si detector, 300-/spl mu/m thick, segmented into 64 /spl times/64 square pixels with 170-/spl mu/m pitch. The solid-state pixel array detector is bump-bonded pixel by pixel to a low threshold, single particle counting electronics, the photon counting chip (PCC) developed by the Medipix1 Collaboration for biomedical applications. Each cell (corresponding to a detector pixel) of the front-end chip has a maximum count rate of 2 MHz, a minimum nominal threshold of 1400 e/sup -/ (corresponding about 5 keV in Si), and contains a 15-bit counter. The detector sensitive area is about 1 cm/sup 2/, but several detectors could be assembled in arrays. The measured background counts were about 3.5/spl times/10/sup -2/ cps/cm/sup 2/, which implies a very high sensitivity of the device. The detection threshold used in this experiment was /spl sim/15 keV. We show the results obtained in the real-time monitoring of two biological dynamic processes: an amino acid uptake by living cells and the thermal denaturation process of oligonucleotides. Specifically, we have followed over time the accumulation of a marked amino acid ([/sup 14/C]L-Leucine) into Octopus vulgaris eggs cells. The results, described in Section III-B, show that after about 20 min from the beginning of the uptake process, the radioactively marked eggs become clearly visible over the background culture radioactive medium. In Sections III-C and III-D, we also describe the successful performance of the /spl beta/-imaging system in genetic studies involving the real time reconstruction of denaturation and kinetics curves for two different /sup 32/P-radiolabeled nucleotides.
- Subjects :
- Physics
Nuclear and High Energy Physics
Pixel
Silicon
business.industry
Detector
Analytical chemistry
chemistry.chemical_element
Autoradiography. Photon counting detectors. Beta rays
equipment and supplies
Kinetic energy
Chip
Photon counting
Semiconductor
Nuclear Energy and Engineering
chemistry
Electronic engineering
sense organs
Electrical and Electronic Engineering
business
Sensitivity (electronics)
Subjects
Details
- ISSN :
- 15581578 and 00189499
- Volume :
- 49
- Database :
- OpenAIRE
- Journal :
- IEEE Transactions on Nuclear Science
- Accession number :
- edsair.doi.dedup.....84fda1be2aa39f5dd3e34728d1670d91