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The role of microRNA-23b-5p in regulating brown adipogenesis and thermogenic program
- Source :
- Endocrine Connections, Endocrine Connections, Vol 9, Iss 5, Pp 457-470 (2020)
- Publication Year :
- 2020
-
Abstract
- Enhanced brown adipose tissue (BAT) mass and activity have been demonstrated to promote the expenditure of excess stored energy and reduce prevalence of obesity. Cold is known as a potent stimulator of BAT and activates BAT primarily through the β3-adrenergic-cAMP signaling. Here, we performed RNA-sequencing to identify differential miRNAs in mouse BAT upon cold exposure and a total of 20 miRNAs were validated. With the treatment of CL-316,243 (CL) and forskolin (Fsk) in mouse and human differentiated brown adipocyte cells in vitro, miR-23b-5p, miR-133a-3p, miR-135-5p, miR-491-5p, and miR-150-3p expression decreased and miR-455-5p expression increased. Among these deferentially expressed miRNAs, miR-23b-5p expression was differentially regulated in activated and aging mouse BAT and negatively correlated with Ucp1 expression. Overexpression of miR-23b-5p in the precursor cells from BAT revealed no significant effects on lipid accumulation, but diminished mitochondrial function and decreased expression of BAT specific markers. Though luciferase reporter assays did not confirm the positive association of miR-23b-5p with the 3′UTRs of the predicted target Ern1, miR-23b-5p overexpression may affect brown adipocyte thermogenic capacity mainly through regulating genes expression involving in lipolysis and fatty acid β-oxidation pathways. Our results suggest that miRNAs are involved in cold-mediated BAT thermogenic activation and further acknowledged miR-23b-5p as a negative regulator in controlling thermogenic programs, further providing potential molecular therapeutic targets to increase surplus energy and treat obesity.
- Subjects :
- 0301 basic medicine
Endocrinology, Diabetes and Metabolism
lcsh:Diseases of the endocrine glands. Clinical endocrinology
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Endocrinology
Precursor cell
microRNA
Brown adipose tissue
Internal Medicine
medicine
Lipolysis
Gene
brown adipose
miR-23b-5p
lcsh:RC648-665
Forskolin
business.industry
Research
Cell biology
ERN1
030104 developmental biology
medicine.anatomical_structure
chemistry
Adipogenesis
lipolysis
β-oxidation
cold stimulation
business
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 20493614
- Volume :
- 9
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Endocrine connections
- Accession number :
- edsair.doi.dedup.....85060c5cb019b23df34de8036c26440c