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Differential action of 3-hydroxyanthranilic acid on viability and activation of stimulated lymphocytes
- Source :
- International Immunopharmacology. 11:2242-2245
- Publication Year :
- 2011
- Publisher :
- Elsevier BV, 2011.
-
Abstract
- article i nfo Lymphocytes proliferation after antigen-driven activation leads to an increase in cell count, which could last some week, until apoptosis mechanisms allow the homeostatic control of the system. During the first days of this stimulation, activated lymphocytes display high resistance to apoptosis and to most immunosuppressive drugs. According to the literature, few compounds have been described to kill recently activated cells, by inhibiting metabolic processes fundamental to proliferation. The aim of our work was to evaluate comparatively these different compounds, in order to identify the best strategy to kill cells that have undergone proliferation, while sparing the repertoire of resting cells. After preliminary experiments, 3-HAA and bortezomib were selected as the most suitable compounds for our purposes. The possible synergic effect of 3-HAA with bortezomib or with manganese ions was also assessed. 3-HAA was confirmed to be the most reliable pharmacologic approach to inhibit proliferation with acceptable toxicity on resting cells. While in the case of PHA stimulation 3-HAA led to death of most lymphocytes, only a minor percentage of cells were killed after allo-stimulation, suggesting that the effect is proportional to the percentage of stimulated lymphocytes. Manganese ions further enhanced this effect, while results with bor- tezomib seemed to be less consistent. These results deserve further investigations to develop new procedures for targeting activated cells with pharmacological approaches.
- Subjects :
- Cell Survival
Immunology
3-hydroxyanthranilic acid
Stimulation
Pharmacology
Biology
Lymphocyte Activation
stimulated lymphocytes
Bortezomib
chemistry.chemical_compound
medicine
Humans
Immunology and Allergy
Lymphocytes
3-Hydroxyanthranilic acid
Cells, Cultured
Manganese
Mixed lymphocyte reaction
Pha stimulation
Boronic Acids
chemistry
Apoptosis
Pyrazines
Toxicity
Homeostasis
medicine.drug
Subjects
Details
- ISSN :
- 15675769
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- International Immunopharmacology
- Accession number :
- edsair.doi.dedup.....85159e803e055e3e23730511e6b81977