Back to Search
Start Over
Influence of salivary conditioning and sucrose concentration on biofilm-mediated enamel demineralization
- Source :
- Journal of Applied Oral Science, Vol 28 (2020), Journal of Applied Oral Science v.28 2020, Journal of applied oral science, Universidade de São Paulo (USP), instacron:USP, Journal of Applied Oral Science
- Publication Year :
- 2020
- Publisher :
- University of São Paulo, 2020.
-
Abstract
- The acquired pellicle formation is the first step in dental biofilm formation. It distinguishes dental biofilms from other biofilm types. Objective To explore the influence of salivary pellicle formation before biofilm formation on enamel demineralization. Methodology Saliva collection was approved by Indiana University IRB. Three donors provided wax–stimulated saliva as the microcosm bacterial inoculum source. Acquired pellicle was formed on bovine enamel samples. Two groups (0.5% and 1% sucrose–supplemented growth media) with three subgroups (surface conditioning using filtered/pasteurized saliva; filtered saliva; and deionized water (DIW)) were included (n=9/subgroup). Biofilm was then allowed to grow for 48 h using Brain Heart Infusion media supplemented with 5 g/l yeast extract, 1 mM CaCl2.2H2O, 5% vitamin K and hemin (v/v), and sucrose. Enamel samples were analyzed for Vickers surface microhardness change (VHNchange), and transverse microradiography measuring lesion depth (L) and mineral loss (∆Z). Data were analyzed using two-way ANOVA. Results The two-way interaction of sucrose concentration × surface conditioning was not significant for VHNchange (p=0.872), ∆Z (p=0.662) or L (p=0.436). Surface conditioning affected VHNchange (p=0.0079), while sucrose concentration impacted ∆Z (p
- Subjects :
- Sucrose
Saliva
Surface Properties
030207 dermatology & venereal diseases
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
stomatognathic system
Hardness
Reference Values
Animals
Yeast extract
Dental Pellicle
Food science
Dental Enamel
Tooth Demineralization
General Dentistry
Enamel paint
Biofilm
RK1-715
030206 dentistry
Salivary pellicle
Microradiography
Demineralization
stomatognathic diseases
chemistry
visual_art
Biofilms
Dentistry
visual_art.visual_art_medium
Brain heart infusion
Dental caries
Pasteurization
Conditioning
Cattle
Original Article
Subjects
Details
- Language :
- English
- ISSN :
- 16787765
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Journal of Applied Oral Science
- Accession number :
- edsair.doi.dedup.....8526dd22b7fb57c38085d5f3168f68b3