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Interleukin 2-induced activation of Ras requires two domains of interleukin 2 receptor beta subunit, the essential region for growth stimulation and Lck-binding domain
- Source :
- The Journal of biological chemistry. 267(35)
- Publication Year :
- 1992
-
Abstract
- Interleukin 2 (IL-2) can stimulate the proliferation of various kinds of T-cell lines. The receptor for IL-2 is composed of at least two subunits (alpha and beta), of which beta subunit plays the major role in transducing growth signals into the cells. A nonreceptor-type tyrosine kinase, Lck, is associated with IL-2 receptor beta subunit, and the binding of IL-2 to its receptor induces the activation of Lck. On the other hand, it has been shown that stimulation of T-cells with IL-2 causes rapid activation of Ras protein. In this paper, we describe that both of the two regions in IL-2 receptor beta subunit, the indispensable region for the induction of cell growth (serine-rich region) and the binding region of Lck protein (acidic region), are required for the activation of Ras. These two regions are also required for tyrosine phosphorylation of an 85-kDa cellular protein (p85) and the accumulation of fos and jun mRNAs. This observation suggests also that the activation of a receptor-associated tyrosine kinase in response to IL-2-stimulation is primarily responsible for subsequent activation of the pathway through Ras to Fos and Jun.
- Subjects :
- Macromolecular Substances
T-Lymphocytes
Interleukin 5 receptor alpha subunit
Biology
Lymphocyte Activation
Transfection
Biochemistry
Guanosine Diphosphate
Models, Biological
Interleukin 10 receptor, alpha subunit
Cell Line
chemistry.chemical_compound
Mice
Genes, jun
Animals
Humans
NCK1
RNA, Messenger
Phosphorylation
Molecular Biology
Interleukin 12 receptor, beta 1 subunit
Interleukin-2 Receptor beta Subunit
Binding Sites
Genes, fos
Tyrosine phosphorylation
Receptors, Interleukin-2
Cell Biology
DNA
Protein-Tyrosine Kinases
Molecular biology
Kinetics
Genes, ras
chemistry
Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
biology.protein
Interleukin-2
Guanosine Triphosphate
Tyrosine kinase
Platelet-derived growth factor receptor
Thymidine
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 267
- Issue :
- 35
- Database :
- OpenAIRE
- Journal :
- The Journal of biological chemistry
- Accession number :
- edsair.doi.dedup.....85c4b42209655bebc4799472f9a50f2d