Back to Search
Start Over
The Protective Effect of the Polysaccharide Precursor, D-Isofloridoside, from Laurencia undulata on Alcohol-Induced Hepatotoxicity in HepG2 Cells
- Source :
- Molecules, Volume 25, Issue 5, Molecules, Vol 25, Iss 5, p 1024 (2020)
- Publication Year :
- 2020
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2020.
-
Abstract
- Alcoholic liver disease (ALD) threatens human health, so it is imperative that we find ways to prevent or treat it. In recent years, the study of polysaccharides has shown that they have different kinds of bioactivities. Among them are many biological effects that have been attributed to polysaccharide precursors. D-Isofloridoside (DIF) is one of the polysaccharide precursors from the marine red alga Laurencia undulata. This study evaluated the effect of DIF on alcohol-induced oxidative stress in human hepatoma cells (HepG2). As a result, DIF attenuated alcohol-induced cytotoxicity, reduced the amount of intracellular reactive oxygen species (ROS), and effectively reduced alcohol-induced DNA damage in HepG2 cells. In addition, a western blot showed that, after DIF treatment, the expression levels of glutathione (GSH), superoxide dismutase (SOD), and B-cell lymphoma-2 (bcl-2) increased, while the expression levels of &gamma<br />glutamyl transferase (GGT), BCL2-associated X (bax), cleaved caspase-3, and mitogen-activated protein kinase (p38 and c-Jun N-terminal kinase ) signal transduction proteins reduced. This showed that DIF may protect cells by reducing the amount of intracellular ROS and inhibiting intracellular oxidative stress and apoptotic processes. Finally, molecular docking demonstrated that DIF can bind to SOD, GGT, B-cell lymphoma-2, and bax proteins. These results indicated that DIF can protect HepG2 cells from alcohol-induced oxidative stress damage, making it an effective potential ingredient in functional foods.
- Subjects :
- DNA damage
Pharmaceutical Science
medicine.disease_cause
Analytical Chemistry
lcsh:QD241-441
Superoxide dismutase
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
lcsh:Organic chemistry
Western blot
Drug Discovery
D-Isofloridoside
medicine
oxidative stress
Physical and Theoretical Chemistry
Protein kinase A
HepG2 cells
030304 developmental biology
0303 health sciences
medicine.diagnostic_test
biology
Chemistry
Organic Chemistry
apoptosis
ROS
Glutathione
Biochemistry
Chemistry (miscellaneous)
030220 oncology & carcinogenesis
biology.protein
Molecular Medicine
Signal transduction
Oxidative stress
Intracellular
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....86331f66690b08d52f1d219b57f223f0
- Full Text :
- https://doi.org/10.3390/molecules25051024