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mRNA GPR162 changes are associated with decreased food intake in rat, and its human genetic variants with impairments in glucose homeostasis in two Swedish cohorts
- Source :
- Gene. 581:139-145
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- G protein-coupled receptors (GPCRs) are a class of integral membrane proteins mediating intercellular interactions of fundamental physiological importance for survival including regulation of food intake, blood pressure, and hormonal sensing signaling, among other roles. Homeostatic alterations in the physiological status of GPCRs are often associated with underlying causes of disease, and to date, several orphan GPCRs are still uncharacterized.Findings from our previous study demonstrate that the Rhodopsin family protein GPR162 is widely expressed in GABAergic as well as other neurons within the mouse hippocampus, whereas extensive expression is observed in hypothalamus, amygdala, and ventral tegmental area, regions strictly interconnected and involved in the regulation of energy homeostasis and hedonic feeding.In this study, we provide a further anatomical characterization of GPR162 in mouse brain via in situ hybridization as well as detailed mRNA expression in a panel of rat tissues complementing a specie-specific mapping of the receptor. We also provide an attempt to demonstrate a functional implication of GPR162 in food intake-related behavior via antisense knockdown studies. Furthermore, we performed human genetic studies in which for the first time, variants of the GPR162 gene were associated with impairments in glucose homeostasis. Fil: Caruso, Vanni. Uppsala University. Department Of Neuroscience. Functional Pharmacology; Suecia. University of Tasmania; Australia Fil: Sheedharan, Smitha. Uppsala University. Department Of Neuroscience. Functional Pharmacology; Suecia Fil: Carlini, Valeria Paola. Uppsala University. Department Of Neuroscience. Functional Pharmacology; Suecia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina Fil: Jacobsson, Josefin A.. Uppsala University. Department Of Neuroscience. Functional Pharmacology; Suecia Fil: Haitina, Tatjana. Uppsala University. Department Of Neuroscience. Functional Pharmacology; Suecia Fil: Hammer, Joanna. Uppsala University. Department Of Neuroscience. Functional Pharmacology; Suecia Fil: Stephansson, Olga. Uppsala University. Department Of Neuroscience. Functional Pharmacology; Suecia Fil: Crona, Filip. Uppsala University. Department Of Neuroscience. Functional Pharmacology; Suecia Fil: Sommer, Wolfgang. Central Institute of Mental Health. Department of Psychopharmacology; Alemania Fil: Risérus, Ulf. Uppsala University. Clinical Nutrition and Metabolism. Department of Public Health and Caring Sciences; Suecia Fil: Lannfelt, Lars. Department Of Neuroscience, Functional Pharmacology; Suecia. Uppsala University. Department of Public Health and Caring Sciences, Geriatrics; Suecia Fil: Marcus, Claude. Department Of Neuroscience, Functional Pharmacology; Suecia. Karolinska Institute. National Childhood Obesity Centre. Department for Clinical Science, Intervention and Technology. Division of Pediatrics; Suecia Fil: Heiling, Markus. National Institutes of Health. National Institute on Alcohol Abuse and Alcoholism. Laboratory of Clinical and Translational Studies; Estados Unidos Fil: Rubiales, Susana Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina Fil: Fredriksson, Robert. Uppsala University. Unit of Functional Pharmacology. Department of Neuroscience ; Suecia Fil: Schiöth, Helgi B.. Uppsala University. Unit of Functional Pharmacology. Department of Neuroscience ; Suecia
- Subjects :
- Male
0301 basic medicine
medicine.medical_specialty
CIENCIAS MÉDICAS Y DE LA SALUD
Adolescent
Ciencias de la Salud
In situ hybridization
Biology
Polymorphism, Single Nucleotide
Energy homeostasis
Receptors, G-Protein-Coupled
Eating
03 medical and health sciences
Food Intake
Gpr162
Species Specificity
Internal medicine
Genetics
medicine
Animals
Homeostasis
Humans
Glucose homeostasis
Gpcrs
Tissue Distribution
Obesity
RNA, Messenger
Rats, Wistar
Child
Receptor
G protein-coupled receptor
Sweden
Gene knockdown
Brain
General Medicine
Middle Aged
humanities
Rats
Cell biology
Otras Ciencias de la Salud
Ventral tegmental area
Glucose
030104 developmental biology
medicine.anatomical_structure
Endocrinology
Female
Glucose Homeostasis
Subjects
Details
- ISSN :
- 03781119
- Volume :
- 581
- Database :
- OpenAIRE
- Journal :
- Gene
- Accession number :
- edsair.doi.dedup.....86470c7ff60468f0cd1185cc440662c9
- Full Text :
- https://doi.org/10.1016/j.gene.2016.01.044