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Invasive neonatal GBS infections from an area-based surveillance study in Italy
- Source :
- Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases. 17(12)
- Publication Year :
- 2011
-
Abstract
- During an area-based study, 75 group B streptococcus (GBS) strains isolated both from early-onset disease (EOD, 37 strains) and from late-onset disease (LOD, 38 strains) were analysed for serotype, pulsed field gel electrophoresis (PFGE) and multilocus sequence typing profiles, protein markers and antibiotic resistance. Serotype III, possessing the rib gene, was the most frequent (54 strains, 72%) and responsible for 89.5% and 54% of LOD and EOD, respectively. Forty-six serotype III strains belonged to the same PFGE type and clonal complex 17, already described as an over-represented clone in neonatal invasive GBS infections. Other serotypes were Ia (9.3%), II (6.7%), Ib (5.3%), V (5.3%) and IV (1.3%). Seventeen PFGE groups were identified comprising strains with related sequence types; conversely, strains displaying the same sequence type could belong to different PFGE groups. When both neonate and maternal strains from vaginorectal swabs and/or milk were available (eight cases), they were indistinguishable. Resistance to erythromycin (12%) was associated with a constitutive resistance to clindamycin in five cases (four carrying the erm(B) gene and one both the erm(B) and mef(E) genes) and with an inducible clindamycin resistance in two cases (one possessing the erm(A) gene, the other the erm(T) gene). Two isolates displayed the M phenotype (mef(E) gene). All strains but five were resistant to tetracycline, mostly mediated by the tet(M) gene (97.1%). The study underlined the importance of an active surveillance system for the elucidation of a GBS population structure causing neonatal infections and allowed the detection of rare antibiotic resistance determinants [erm(T)].
- Subjects :
- Serotype
antibiotic resistance
Proteome
Drug resistance
medicine.disease_cause
S. agalactiae
Cluster Analysis
Alpha-like protein family
Antibiotic resistance
Group B streptococcus
MLST
Molecular epidemiology
Neonatal infection
PFGE
Anti-Bacterial Agents
Bacterial Proteins
Clindamycin
DNA Fingerprinting
DNA, Bacterial
Drug Resistance, Bacterial
Electrophoresis, Gel, Pulsed-Field
Erythromycin
Genotype
Humans
Infant
Infant, Newborn
Italy
Microbial Sensitivity Tests
Molecular Epidemiology
Multilocus Sequence Typing
Phenotype
Serotyping
Streptococcal Infections
Streptococcus agalactiae
Tetracycline
Microbiology (medical)
Infectious Diseases
serotype
General Medicine
medicine.drug
group B streptococcus
Biology
Microbiology
Pulsed-field gel electrophoresis
medicine
neonatal infection
biochemical phenomena, metabolism, and nutrition
Virology
Multilocus sequence typing
Subjects
Details
- ISSN :
- 14690691
- Volume :
- 17
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
- Accession number :
- edsair.doi.dedup.....866236440cdee3e3fd3e0689007574cb