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Rapid hyperspectral fluorescence lifetime imaging
- Source :
- Microscopy Research and Technique. 70:481-484
- Publication Year :
- 2007
- Publisher :
- Wiley, 2007.
-
Abstract
- We report a rapid hyperspectral fluorescence lifetime imaging (FLIM) instrument that exploits high-speed FLIM technology in a line-scanning microscope. We demonstrate the acquisition of whole-field optically sectioned hyperspectral fluorescence lifetime image stacks (with 32 spectral bins) in less than 40 s and illustrate its application to unstained biological tissue. Microsc. Res. Tech., 2007. © 2007 Wiley-Liss, Inc.
- Subjects :
- Diagnostic Imaging
Fluorescence-lifetime imaging microscopy
Time Factors
Histology
Materials science
Microscope
business.industry
Hyperspectral imaging
Arteries
Biological tissue
Fluorescence
Convallaria
law.invention
Medical Laboratory Technology
Optics
Microscopy, Fluorescence
law
Medical imaging
Humans
Anatomy
Tissue autofluorescence
business
Instrumentation
Subjects
Details
- ISSN :
- 10970029 and 1059910X
- Volume :
- 70
- Database :
- OpenAIRE
- Journal :
- Microscopy Research and Technique
- Accession number :
- edsair.doi.dedup.....867363726d3d0fcb60a1fc3e278e1388
- Full Text :
- https://doi.org/10.1002/jemt.20434