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Quantitative paper-based dot blot assay for spike protein detection using fuchsine dye-loaded polymersomes
- Source :
- Biosensors and Bioelectronics. 192:113484
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Real-time reverse transcriptase-polymerase chain reaction (RT-PCR)-based assays are the gold standard for virus diagnosis. Point-of-care (POC) technologies have shown great progress during this period. Herein, we propose a novel fuchsine dye-loaded polymersome for a colorimetric paper-based dot blot spike protein diagnostic assay for COVID-19 via smartphone-assisted sensing. The prepared platform aimed to create an adaptable tool that competes with traditional nanoparticle-based assays employing gold and silver. Analytical characterization and application of the testing platform showed high sensitivity (10 times better than gold nanoparticles), stability, fast turnaround, and reproducibility. The potential and possibilities demonstrated by the current platform could be observed in its adaptability for different markers and pathologies. In addition, smartphone-assisted sensing emphasizes the ability to use the tool at home by common peoples which can lower the burden on the healthcare facilities and reach more underdeveloped regions.<br />Research Foundation of Ege University, Turkey [TOA-2020-21862]; Republic of Turkey, Ministry of Development [2010K120810/2020K12150700, 2016K121190]<br />The research project was supported by the Research Foundation of Ege University, Turkey (Project number: TOA-2020-21862) . Republic of Turkey, Ministry of Development provided the support for the infra-structure of EGE MATAL (Ege University/Izmir) via 2010K120810/2020K12150700 and 2016K121190 grants.
- Subjects :
- Fuchsine
Coronavirus disease 2019 (COVID-19)
Biomedical Engineering
Biophysics
Amplification
Metal Nanoparticles
Dot blot
Paper-based colorimetric assay
Nanotechnology
Biosensing Techniques
Biofunctionalization
Care
Sensitivity and Specificity
chemistry.chemical_compound
Rosaniline Dyes
Electrochemistry
Dye-loaded polymersome
Humans
Antibody
SARS-CoV-2
COVID-19
Reproducibility of Results
Spike Protein
General Medicine
Gold standard (test)
Technologies
chemistry
In-Vitro
Colloidal gold
Spike Glycoprotein, Coronavirus
Polymersome
Gold
Lateral Flow Assay
Biosensor
Biotechnology
Subjects
Details
- ISSN :
- 09565663
- Volume :
- 192
- Database :
- OpenAIRE
- Journal :
- Biosensors and Bioelectronics
- Accession number :
- edsair.doi.dedup.....86eb081eec6513a1f5331fe2aa5e4be8