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Integration of transcriptional and mutational data simplifies the stratification of peripheral T‐cell lymphoma
- Source :
- American Journal of Hematology. 94:628-634
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- © 2019 Wiley Periodicals, Inc. The histological diagnosis of peripheral T-cell lymphoma (PTCL) can represent a challenge, particularly in the case of closely related entities such as angioimmunoblastic T-lymphoma (AITL), PTCL-not otherwise specified (PTCL-NOS), and ALK-negative anaplastic large-cell lymphoma (ALCL). Although gene expression profiling and next generations sequencing have been proven to define specific features recurrently associated with distinct entities, genomic-based stratifications have not yet led to definitive diagnostic criteria and/or entered into the routine clinical practice. Herein, to improve the current molecular classification between AITL and PTCL-NOS, we analyzed the transcriptional profiles from 503 PTCLs stratified according to their molecular configuration and integrated them with genomic data of recurrently mutated genes (RHOA G17V , TET2, IDH2 R172 , and DNMT3A) in 53 cases (39 AITLs and 14 PTCL-NOSs) included in the series. Our analysis unraveled that the mutational status of RHOA G17V , TET2, and DNMT3A poorly correlated, individually, with peculiar transcriptional fingerprints. Conversely, in IDH2 R172 samples a strong transcriptional signature was identified that could act as a surrogate for mutational status. The integrated analysis of clinical, mutational, and molecular data led to a simplified 19-gene signature that retains high accuracy in differentiating the main nodal PTCL entities. The expression levels of those genes were confirmed in an independent cohort profiled by RNA-sequencing.
- Subjects :
- Male
medicine.medical_specialty
RHOA
Transcription, Genetic
Computational biology
IDH2
Article
peripheral T‐cell lymphoma
03 medical and health sciences
0302 clinical medicine
Internal medicine
gene expression profiling
medicine
Humans
Gene
Regulation of gene expression
Hematology
Hematology, peripheral T‐cell lymphoma, gene expression profiling, molecular classification, IDH2
molecular classification
biology
peripheral T-cell lymphoma, mutational status
Lymphoma, T-Cell, Peripheral
medicine.disease
Peripheral T-cell lymphoma
peripheral T-cell lymphoma, gene expression profiling,Stratification,Mutational Data
Neoplasm Proteins
Lymphoma
Gene Expression Regulation, Neoplastic
Gene expression profiling
030220 oncology & carcinogenesis
Mutation
biology.protein
Female
030215 immunology
Subjects
Details
- ISSN :
- 10968652 and 03618609
- Volume :
- 94
- Database :
- OpenAIRE
- Journal :
- American Journal of Hematology
- Accession number :
- edsair.doi.dedup.....8714bf0ba12ef92760256906dcede5e9