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Molecular characterization of the insecticidal activity of double-stranded RNA targeting the smooth septate junction of western corn rootworm (Diabrotica virgifera virgifera)
- Source :
- PLoS ONE, Vol 14, Iss 1, p e0210491 (2019), PLoS ONE
- Publication Year :
- 2019
- Publisher :
- Public Library of Science (PLoS), 2019.
-
Abstract
- The western corn rootworm (WCR, Diabrotica virgifera virgifera) gene, dvssj1, is a putative homolog of the Drosophila melanogaster gene, snakeskin (ssk). This gene encodes a membrane protein associated with the smooth septate junction (SSJ) which is required for the proper barrier function of the epithelial lining of insect intestines. Disruption of DVSSJ integrity by RNAi technique has been shown previously to be an effective approach for corn rootworm control, by apparent suppression of production of DVSSJ1 protein leading to growth inhibition and mortality. To understand the mechanism that leads to the death of WCR larvae by dvssj1 double-stranded RNA, we examined the molecular characteristics associated with SSJ functions during larval development. Dvssj1 dsRNA diet feeding results in dose-dependent suppression of mRNA and protein; this impairs SSJ formation and barrier function of the midgut and results in larval mortality. These findings suggest that the malfunctioning of the SSJ complex in midgut triggered by dvssj1 silencing is the principal cause of WCR death. This study also illustrates that dvssj1 is a midgut-specific gene in WCR and its functions are consistent with biological functions described for ssk.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Insecticides
Life Cycles
Molecular biology
Genes, Insect
01 natural sciences
Biochemistry
Binding Analysis
Larvae
RNA interference
Medicine and Health Sciences
Drosophila Proteins
Small interfering RNAs
Multidisciplinary
biology
Drosophila Melanogaster
Eukaryota
Animal Models
Cell biology
Coleoptera
Nucleic acids
Insects
RNA silencing
RNA isolation
Experimental Organism Systems
Larva
Insect Proteins
Medicine
RNA Interference
Drosophila
Drosophila melanogaster
Cell Binding Assay
Research Article
Arthropoda
Science
Research and Analysis Methods
Biomolecular isolation
Insect Control
Zea mays
03 medical and health sciences
Model Organisms
Genetics
Gene silencing
Animals
RNA, Messenger
Pest Control, Biological
Non-coding RNA
Gene
Immunohistochemistry Techniques
Chemical Characterization
RNA, Double-Stranded
Nutrition
Biology and life sciences
Smooth septate junction
fungi
Organisms
Membrane Proteins
Midgut
biology.organism_classification
Invertebrates
Gene regulation
Diet
Histochemistry and Cytochemistry Techniques
010602 entomology
030104 developmental biology
Western corn rootworm
Molecular biology techniques
Animal Studies
Immunologic Techniques
RNA
Gene expression
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 14
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....872866cb857f960b3d7c6a7abbf84832