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miR-26a is Involved in Glycometabolism and Affects Boar Sperm Viability by Targeting PDHX
- Source :
- Cells, Cells, Vol 9, Iss 1, p 146 (2020), Volume 9, Issue 1
- Publication Year :
- 2020
- Publisher :
- MDPI, 2020.
-
Abstract
- miR-26a is associated with sperm metabolism and can affect sperm motility and apoptosis. However, how miR-26a affects sperm motility remains largely unknown. Our previous study indicated that the PDHX gene is predicted to be a potential target of miR-26a, which is responsible for pyruvate oxidative decarboxylation which is considered as a key step for connecting glycolysis with oxidative phosphorylation. In this study, we first reported a potential relationship between miR-26a and PDHX and their expressions in fresh, frozen-thawed, and epididymal boar sperm. Then, sperm viability and survival were determined after transfection of miR-26a. mRNA and protein expression level of PDHX in the liquid-preserved boar sperm after transfection were also determined by RT-qPCR and Western Blot (WB). Our results showed that expression level of PDHX was significantly increased during sperm transit from epididymal caput to corpus and cauda. Similarly, expression of PDHX was significantly higher (P &lt<br />0.05) in fresh sperm as compared to epididymal cauda and frozen-thawed sperm. However, the expression of miR-26a in epididymal corpus sperm was significantly higher (P &lt<br />0.05) than that of caput and cauda sperm. Furthermore, after transfection of boar sperm with miR-26a mimic and inhibitor under liquid storage, the lowest and highest sperm viability was observed in miR-26a mimic and inhibitor treatment (P &lt<br />0.05), respectively. The protein levels of PDHX, after 24 and 48 h of transfection of miR-26a mimics and inhibitor, were notably decreased and increased (P &lt<br />0.05), respectively, as compared to negative control (NC) group. In conclusion, the novel and enticing findings of our study provide a reasonable evidence that miR-26a via PDHX, a link between glycolysis and oxidative phosphorylation, could regulate the glycometabolic pathway which eventually affect boar sperm viability and survival.
- Subjects :
- 0301 basic medicine
Male
endocrine system
miR-26a
Cell Survival
Swine
glycometabolism
Pyruvate Dehydrogenase Complex
Oxidative phosphorylation
boar sperm
sperm viability
Article
Andrology
pdhx
03 medical and health sciences
0302 clinical medicine
Western blot
medicine
Animals
Glycolysis
lcsh:QH301-705.5
Sperm motility
reproductive and urinary physiology
030219 obstetrics & reproductive medicine
medicine.diagnostic_test
Chemistry
urogenital system
General Medicine
Transfection
Metabolism
Sperm
Spermatozoa
MicroRNAs
030104 developmental biology
lcsh:Biology (General)
Gene Expression Regulation
Apoptosis
Sperm Motility
PDHX
Subjects
Details
- Language :
- English
- ISSN :
- 20734409
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Cells
- Accession number :
- edsair.doi.dedup.....872d9171ea5e468fa5dffd8ec1b51420