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Preparation of Biomolecule Microstructures and Microarrays by Thiol-ene Photoimmobilization
- Source :
- ChemBioChem, 11(2), 235-247. Wiley-VCH Verlag, ChemBioChem, 11, 235-247. Wiley-VCH Verlag, ChemBioChem, 11, 2, pp. 235-247, ChemBioChem, 11, 235-247
- Publication Year :
- 2009
- Publisher :
- Wiley, 2009.
-
Abstract
- A mild, fast and flexible method for photoimmobilization of biomolecules based on the light-initiated thiol-ene reaction has been developed. After investigation and optimization of various surface materials, surface chemistries and reaction parameters, microstructures and microarrays of biotin, oligonucleotides, peptides, and MUC1 tandem repeat glycopeptides were prepared with this photoimmobilization method. Furthermore, MUC1 tandem repeat glycopeptide microarrays were successfully used to probe antibodies in mouse serum obtained from vaccinated mice. Dimensions of biomolecule microstructures were shown to be freely controllable through photolithographic techniques, and features down to 5 microm in size covering an area of up to 75x25 mm were created. Use of a confocal laser microscope with a UV laser as UV-light source enabled further reduction of biotin feature size opening access to nanostructured biochips.
- Subjects :
- Light
Ultraviolet Rays
Microarrays
Oligonucleotides
Biotin
Nanotechnology
Correlated Electron Systems / High Field Magnet Laboratory (HFML)
Biochemistry
Antibodies
Mice
chemistry.chemical_compound
Tandem repeat
IR-72760
Animals
Sulfhydryl Compounds
Biochip
Molecular Biology
Ene reaction
chemistry.chemical_classification
thiol–ene reaction
photochemistry
Thiol-ene reaction
Oligonucleotide
Biomolecule
Mucin-1
Organic Chemistry
Glycopeptides
Microarray Analysis
Photochemical Processes
Immobilized Proteins
Biochips
chemistry
immobilization
Molecular Medicine
DNA microarray
METIS-273430
Subjects
Details
- ISSN :
- 14394227
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- ChemBioChem
- Accession number :
- edsair.doi.dedup.....872f7bb624f5a7e59db58bd0c7645173