Back to Search
Start Over
Prevalence and Distribution of High-Risk Human Papillomavirus Genotypes in Invasive Carcinoma of the Uterine Cervix in Uruguay
- Source :
- International Journal of Gynecological Cancer, International Journal of Gynecological Cancer, Lippincott, Williams & Wilkins, 2013, 23 (3), pp.527-32. ⟨10.1097/IGC.0b013e318285e753⟩
- Publication Year :
- 2013
- Publisher :
- BMJ, 2013.
-
Abstract
- ObjectivesPersistent infection with specific genotypes of human papillomaviruses (HPVs) is the main cause of invasive cervical cancer (ICC). Only a few of the various HPV types account for most of the cases worldwide, and geographical differences in their distribution are evident. Data from locally prevalent genotypes are essential in view of introduction of HPV type-specific prophylactic vaccines.MethodsIn this work, we have investigated HPV type distribution in samples of ICC cases that occurred in Uruguayan women. DNA extracted from ICC treated in Centro Hospitalario Pereira Rossell of Montevideo between 1999 and 2007 were analyzed. Search and typing were performed by polymerase chain reaction using generic GP5+/GP6+ primers and specific primers for HPV types 16, 18, 33, and 45. Positive GP5+/GP6+ samples, which were negative for all 4 high-risk HPV-specific types screened were further analyzed by sequencing.ResultsHuman papillomavirus DNA sequences were found in 163 (92.6%) of 176 cases. The most prevalent genotypes were HPV16 (67.6%) and HPV18 (8.5%) followed by HPV45 (6.8%) and HPV33 (3.4%), as single or mixed infection. Other less frequent genotypes were HPV31, HPV35, HPV39, HPV51, HPV52, HPV58, HPV66, and HPV73. The viral type could not be determined (HPV X) in 1 case (0.6%) of the HPV DNA–positive cervical cancers and double infections were found in 1.7% of the cases. The higher percentage of most aggressive HPV (16/18/45) genotypes was detected in cases diagnosed at younger than 60 years old, whereas these genotypes were less frequent in older patients.ConclusionWe conclude that HPV types 16, 18, and 45 have a very high prevalence in ICC of Uruguayan women. Results provide evidence that 16 of 18 infections are more aggressive, but most cancers could be vaccine preventable.
- Subjects :
- [SDV]Life Sciences [q-bio]
Uterine Cervical Neoplasms
Gastroenterology
law.invention
0302 clinical medicine
Risk Factors
law
Genotype
Prevalence
Papillomaviridae
Polymerase chain reaction
0303 health sciences
High prevalence
Invasive carcinoma
Hpv types
Obstetrics and Gynecology
Human papillomavirus genotypes
Middle Aged
Prognosis
3. Good health
Oncology
030220 oncology & carcinogenesis
Carcinoma, Squamous Cell
Female
Adult
medicine.medical_specialty
Adenocarcinoma
Young Adult
03 medical and health sciences
Internal medicine
medicine
Invasive cervical cancer
Humans
Neoplasm Invasiveness
Typing
Human papillomavirus
Retrospective Studies
030304 developmental biology
Gynecology
business.industry
Papillomavirus Infections
Uterine Cervical Dysplasia
Cross-Sectional Studies
Uterine cervix
DNA, Viral
Uruguay
Neoplasm Grading
business
Follow-Up Studies
Subjects
Details
- ISSN :
- 15251438 and 1048891X
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- International Journal of Gynecologic Cancer
- Accession number :
- edsair.doi.dedup.....8743c5a1856a21defd98ea6a275698d0