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Copper redistribution in murine macrophages in response to Salmonella infection
- Source :
- The Biochemical journal. 444(1)
- Publication Year :
- 2012
-
Abstract
- The movement of key transition metal ions is recognized to be of critical importance in the interaction between macrophages and intracellular pathogens. The present study investigated the role of copper in mouse macrophage responses to Salmonella enterica sv. Typhimurium. The copper chelator BCS (bathocuproinedisulfonic acid, disodium salt) increased intracellular survival of S. Typhimurium within primary mouse BMM (bone-marrow-derived macrophages) at 24 h post-infection, implying that copper contributed to effective host defence against this pathogen. Infection of BMM with S. Typhimurium or treatment with the TLR (Toll-like receptor) 4 ligand LPS (lipopolysaccharide) induced the expression of several genes encoding proteins involved in copper transport [Ctr (copper transporter) 1, Ctr2 and Atp7a (copper-transportingATPase 1)], as well as the multi-copper oxidase Cp (caeruloplasmin). Both LPS and infectionwith S. Typhimurium triggered copper accumulation within punctate intracellular vesicles (copper 'hot spots') in BMM as indicated by the fluorescent reporter CS1 (copper sensor 1). These copper hot spots peaked in their accumulation at approximately 18 h post-stimulation and were dependent on copper uptake into cells. Localization studies indicated that the copper hot spotswere in discrete vesicles distinct from Salmonella containing vacuoles and lysosomes. We propose that copper hot spot formation contributes to antimicrobial responses against professional intracellular bacterial pathogens. © The Authors Journal compilation © 2012 Biochemical Society.
- Subjects :
- Boron Compounds
Lipopolysaccharides
Male
Salmonella typhimurium
Biochemistry & Molecular Biology
Lipopolysaccharide
Cations, Divalent
ATP7A
chemistry.chemical_element
Vacuole
Biology
Sulfides
Biochemistry
Microbiology
Copper-transporting ATPases
chemistry.chemical_compound
Mice
Metalloproteins
Animals
Homeostasis
Molecular Biology
Cation Transport Proteins
Copper Transporter 1
Fluorescent Dyes
Adenosine Triphosphatases
Intracellular parasite
Macrophages
Ceruloplasmin
Cell Biology
biology.organism_classification
Copper
Mice, Inbred C57BL
chemistry
Salmonella enterica
Copper-Transporting ATPases
Salmonella Infections
biology.protein
Intracellular
Subjects
Details
- ISSN :
- 14708728
- Volume :
- 444
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- The Biochemical journal
- Accession number :
- edsair.doi.dedup.....877e2916bd7808fe8bd3ed170aa3f955