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Expression, purification, crystallization and preliminary crystallographic analysis of SpaA, a major pilin fromCorynebacterium diphtheriae
- Source :
- Acta Crystallographica Section F Structural Biology and Crystallization Communications. 65:802-804
- Publication Year :
- 2009
- Publisher :
- International Union of Crystallography (IUCr), 2009.
-
Abstract
- Bacterial pili are cell-surface organelles that are critically involved in adhesion to host cells, leading to the colonization of host tissues and the establishment of infections. Whereas the pili of Gram-negative bacteria have been extensively studied, those of Gram-positive bacteria came to light only recently after the discovery and characterization of Corynebacterium diphtheriae pili. These newly discovered pili are formed by the covalent polymerization of pilin subunits catalyzed by sortase enzymes, making them fundamentally different from the noncovalent pilin assemblies of Gram-negative bacteria. Here, the expression, crystallization and preliminary crystallographic analysis of SpaA, which forms the shaft of one of the three types of pili expressed by C. diphtheriae, are reported. SpaA(53-486) crystals diffracted to 1.6 A resolution and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 34.9, b = 64.1, c = 198.7 A, alpha = beta = gamma = 90 degrees .
- Subjects :
- Biophysics
Crystallography, X-Ray
Biochemistry
Pilus
Fimbriae Proteins
Microbiology
Bacterial Proteins
Structural Biology
Sortase
Organelle
Genetics
Cloning, Molecular
DNA Primers
chemistry.chemical_classification
Corynebacterium diphtheriae
Base Sequence
biology
biochemical phenomena, metabolism, and nutrition
Condensed Matter Physics
biology.organism_classification
Crystallography
Enzyme
chemistry
Crystallization Communications
Pilin
biology.protein
bacteria
Crystallization
Bacteria
Subjects
Details
- ISSN :
- 17443091
- Volume :
- 65
- Database :
- OpenAIRE
- Journal :
- Acta Crystallographica Section F Structural Biology and Crystallization Communications
- Accession number :
- edsair.doi.dedup.....8792bdb67ec8edcb2434089c6b807b99
- Full Text :
- https://doi.org/10.1107/s1744309109027596