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Expression, purification, crystallization and preliminary crystallographic analysis of SpaA, a major pilin fromCorynebacterium diphtheriae

Authors :
Neil G. Paterson
Hae Joo Kang
Edward N. Baker
Source :
Acta Crystallographica Section F Structural Biology and Crystallization Communications. 65:802-804
Publication Year :
2009
Publisher :
International Union of Crystallography (IUCr), 2009.

Abstract

Bacterial pili are cell-surface organelles that are critically involved in adhesion to host cells, leading to the colonization of host tissues and the establishment of infections. Whereas the pili of Gram-negative bacteria have been extensively studied, those of Gram-positive bacteria came to light only recently after the discovery and characterization of Corynebacterium diphtheriae pili. These newly discovered pili are formed by the covalent polymerization of pilin subunits catalyzed by sortase enzymes, making them fundamentally different from the noncovalent pilin assemblies of Gram-negative bacteria. Here, the expression, crystallization and preliminary crystallographic analysis of SpaA, which forms the shaft of one of the three types of pili expressed by C. diphtheriae, are reported. SpaA(53-486) crystals diffracted to 1.6 A resolution and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 34.9, b = 64.1, c = 198.7 A, alpha = beta = gamma = 90 degrees .

Details

ISSN :
17443091
Volume :
65
Database :
OpenAIRE
Journal :
Acta Crystallographica Section F Structural Biology and Crystallization Communications
Accession number :
edsair.doi.dedup.....8792bdb67ec8edcb2434089c6b807b99
Full Text :
https://doi.org/10.1107/s1744309109027596