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Gain of imprinting at chromosome 11p15: a pathogenetic mechanism identified in human hepatocarcinomas
- Publication Year :
- 2000
-
Abstract
- Genomic imprinting is a reversible condition that causes parental-specific silencing of maternally or paternally inherited genes. Analysis of DNA and RNA from 52 human hepatocarcinoma samples revealed abnormal imprinting of genes located at chromosome 11p15 in 51% of 37 informative samples. The most frequently detected abnormality was gain of imprinting, which led to loss of expression of genes present on the maternal chromosome. As compared with matched normal liver tissue, hepatocellular carcinomas showed extinction or significant reduction of expression of one of the alleles of the CDKN1C , SLC22A1L , and IGF2 genes. Loss of maternal-specific methylation at the KvDMR1 locus in hepatocarcinoma correlated with abnormal expression of CDKN1C and IGF2 , suggesting a function for KvDMR1 as a long-range imprinting center active in adult tissues. These results point to the role of epigenetic mechanisms leading to loss of expression of imprinted genes at chromosome region 11p15 in human tumors.
- Subjects :
- Adult
Cyclin-Dependent Kinase Inhibitor p21
Male
Heterozygote
Carcinoma, Hepatocellular
Locus (genetics)
Biology
Genomic Imprinting
Insulin-Like Growth Factor II
Cyclins
Humans
Epigenetics
Gene Silencing
Imprinting (psychology)
Gene
Genetics
Regulation of gene expression
Multidisciplinary
Chromosomes, Human, Pair 11
Homozygote
Liver Neoplasms
Chromosome
Chromosome Mapping
DNA Methylation
Biological Sciences
Molecular biology
Gene Expression Regulation, Neoplastic
DNA methylation
Female
Genes, MDR
Genomic imprinting
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....87abacd0394f7d3f711f615852e70acf