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Oxidized low-density lipoprotein increases interleukin-8 production in human gingival epithelial cell line Ca9-22
- Source :
- Journal of periodontal research. 45(4)
- Publication Year :
- 2010
-
Abstract
- Suzuki K, Sakiyama Y, Usui M, Obama T, Kato R, Itabe H, Yamamoto M. Oxidized low-density lipoprotein increases interleukin-8 production in human gingival epithelial cell line Ca9-22. J Periodont Res 2010; 45: 488–495. © 2010 John Wiley & Sons A/S Background and Objective: Recent epidemiological studies have shown a correlation between periodontitis and hyperlipidemia. We have found high levels of oxidized low-density lipoprotein (OxLDL) in the gingival crevicular fluid of dental patients. In the present study, we tried to examine the possible role of OxLDL in periodontal inflammation in vitro. Material and Methods: Cells of the human gingival epithelial cell line Ca9-22 were cultured in media containing OxLDL, and the amounts of interleukin-8 (IL-8) and prostaglandin E2 (PGE2) produced were measured using ELISAs. Results: Production of IL-8 by Ca9-22 cells was significantly increased when the cells were treated with OxLDL, but not with native LDL or acetylated LDL. Production of PGE2 by Ca9-22 cells was enhanced by co-incubation with OxLDL and interleukin-1β (IL-1β). Scavenger receptor inhibitors, fucoidan and dextran sulfate, inhibited the OxLDL-induced IL-8 and PGE2 production in the presence of IL-1β. The p38 MAPK inhibitors SB203580 and SB202190 and the ERK inhibitor PD98059 inhibited the OxLDL-induced IL-8 production. Among oxidized lipids and chemically modified LDL, 7-ketocholesterol enhanced IL-8 production. Conclusion: This is the first report to show that OxLDL enhances IL-8 production in epithelial cells.
- Subjects :
- MAPK/ERK pathway
Pyridines
Interleukin-1beta
Gingiva
Sulfuric Acid Esters
p38 Mitogen-Activated Protein Kinases
Dinoprostone
chemistry.chemical_compound
Polysaccharides
Cell Line, Tumor
Humans
Interleukin 8
Scavenger receptor
Enzyme Inhibitors
Receptor
Cholesterol 7-alpha-Hydroxylase
Periodontitis
Ketocholesterols
Chemokine CCL2
Fucose
Flavonoids
Receptors, Scavenger
Fucoidan
Dextran Sulfate
Interleukin-8
Imidazoles
Epithelial Cells
Molecular biology
In vitro
Lipoproteins, LDL
Biochemistry
chemistry
Cell culture
Periodontics
lipids (amino acids, peptides, and proteins)
Mitogen-Activated Protein Kinases
Oxidation-Reduction
Lipoprotein
Subjects
Details
- ISSN :
- 16000765
- Volume :
- 45
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Journal of periodontal research
- Accession number :
- edsair.doi.dedup.....87b040ba12a3353069b85d1271c93647