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KCC3 deficiency-induced disruption of paranodal loops and impairment of axonal excitability in the peripheral nervous system
- Source :
- Neuroscience. 335:91-102
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- The autosomal recessive Hereditary Motor and Sensory Neuropathy with Agenesis of the Corpus Callosum (HMSN/ACC) is associated with the dysfunction of the K + –Cl − cotransporter type 3 (KCC3), which is an electroneutral cotransporter. We previously found that the inhibition of KCC3 cotransporter activity reduces the propagation of action potentials in the peripheral nervous system (PNS). However, the pathogenesis by which KCC3 deficiency impairs peripheral nerve function remains to be examined. Thus, we conducted imaging and electrophysiological studies in the peripheral nerves of KCC3 −/− mice at various ages. Analysis using transmission electron microscopy (TEM) revealed an age-dependent progressive swelling of microvilli and disorganization of paranodal loops in KCC3 −/− nerves. Yet, no mislocated voltage-dependent channels were observed between the nodes and juxtaparanodes of KCC3 −/− nerves. However, electrophysiological studies using the threshold tracking technique indicated a reduced stimulus–response curve slope with an elevated rheobase, a decreased strength–duration time constant, diminished persistent Na + currents, and an outward deviation of threshold electrotonus in KCC3 −/− nerves compared to wild-type nerves. These functional changes indicate an overall reduction in axonal excitability and suggest an increase in paranodal conductance, which was relevant to the pathology at the paranode. Altogether, our findings highlight the importance of KCC3 in maintaining paranodal integrity and in optimizing the propagation of action potentials along peripheral nerves.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Genotype
Action Potentials
Pathogenesis
03 medical and health sciences
0302 clinical medicine
Internal medicine
Peripheral Nervous System
medicine
Animals
Agenesis of the corpus callosum
Mice, Knockout
Symporters
Chemistry
General Neuroscience
medicine.disease
Axons
Peripheral
Electrophysiology
030104 developmental biology
Endocrinology
Rheobase
medicine.anatomical_structure
Peripheral nervous system
Cotransporter
Hereditary motor and sensory neuropathy
Neuroscience
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 03064522
- Volume :
- 335
- Database :
- OpenAIRE
- Journal :
- Neuroscience
- Accession number :
- edsair.doi.dedup.....87c8f1c4b12190a86c496a51902df0d1