Back to Search Start Over

Application of the Co-culture Membrane System Pointed to a Protective Role of Catestatin on Hippocampal Plus Hypothalamic Neurons Exposed to Oxygen and Glucose Deprivation

Authors :
Marcello Canonaco
Loredana De Bartolo
Ennio Avolio
Gilda Fazzari
Rosa Maria Facciolo
Sabrina Morelli
Maria Mele
Antonella Piscioneri
Raffaella Alò
Source :
Molecular neurobiology. 54(9)
Publication Year :
2016

Abstract

Depletion of oxygen and glucose even for brief periods is sufficient to cause cerebral ischemia, which is a predominant worldwide cause of motor deficits with the reduction of life quality and subsequently death. Hence, more insights regarding protective measures against ischemic events are becoming a major research goal. Among the many neuronal factors, N-methyl-D-aspartate receptors (NMDAR), orexinergic neuroreceptors (ORXR), and sympatho-inhibitory neuropeptide catestatin (CST) are widely involved with ischemic episodes. In this study, it was possible to induce in vitro ischemic conditions of the hamster (Mesocricetus auratus) hippocampal and hypothalamic neuronal cultures, grown on a newly compartmentalized membrane system, via oxygen and glucose deprivation (OGD). These cultures displayed notably differentiated NMDARergic and ORXergic receptor expression activities along with evident brain-derived neurotrophic factor (BDNF) plus orexin A (ORX-A) secretion, especially under co-cultured conditions. Interestingly, addition of CST in OGD-insulted hippocampal cells accounted for upregulated GluN1 and ORX1R transcripts that in the case of the latter neuroreceptor was very strongly (p

Details

ISSN :
15591182
Volume :
54
Issue :
9
Database :
OpenAIRE
Journal :
Molecular neurobiology
Accession number :
edsair.doi.dedup.....87e0fa9a85956676db8f65a87cd5e452