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The role of delta-1-pyrroline-5-carboxylate dehydrogenase (P5CDh) in the Pacific white shrimp (Litopenaeus vannamei) during biotic and abiotic stress
- Source :
- Aquatic Toxicology. 208:1-11
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Proline (Pro) metabolism is intimately associated with stress adaptation. The catabolism of Pro includes two dehydrogenation reactions catalyzed by proline dehydrogenase (ProDH) and Δ1-pyrroline-5-carboxylate dehydrogenase (P5CDh). P5CDh is a mitochondrial matrix NAD+-dependent dehydrogenase that is critical in preventing P5C-Pro intensive cycling and avoiding ROS production from electron run-off. Little is known about the roles of P5CDh in invertebrates, however. We cloned the P5CDh sequence in the Pacific white shrimp, Litopenaeus vannamei, and found that LvP5CDh is expressed predominantly in pleopod, hepatopancreas and gill. Subcellular localization analysis revealed that LvP5CDh protein was mainly found in the cytoplasm. In addition, overexpressing LvP5CDh in cells reduced ROS formation and inhibited apoptosis induced by LC50 Cd2+. Shrimp were exposed to various stress factors including infection with Vibrio alginolyticus, (½ LC50 and LC50) Cd2+, acid (pH 5.6) and alkali stress (pH 9.3). Both biotic and abiotic stress resulted in increased LvP5CDh expression and Pro accumulation; V. alginolyticus infection, pH 9.3 and LC50 Cd2+ stress apparently stimulated the Glu pathway of Pro synthesis, while pH 5.6 and ½ LC50 Cd2+ stress promoted the Orn pathway of Pro synthesis. Silencing of Lvp53 in shrimp attenuated LvP5CDh expression during Cd2+ stress, but had no effect on LvP5CDh mRNA levels if no Cd2+ stress was imposed. Our study contributes to the functional characterization of LvP5CDh in biotic and abiotic stress and reveals it to protect against ROS generation, damage to the cell, including the mitochondria, and apoptosis. Thus, LvP5CDh plays a critical role in immune defense and antioxidant responses.
- Subjects :
- DNA, Complementary
Proline
Health, Toxicology and Mutagenesis
Glutamic Acid
Apoptosis
Dehydrogenase
010501 environmental sciences
Aquatic Science
01 natural sciences
Antibodies
03 medical and health sciences
Proline dehydrogenase
Penaeidae
Stress, Physiological
Animals
Amino Acid Sequence
Gene Silencing
030304 developmental biology
0105 earth and related environmental sciences
Vibrio alginolyticus
0303 health sciences
biology
Chemistry
Abiotic stress
Reproducibility of Results
biology.organism_classification
Recombinant Proteins
Mitochondria
Shrimp
1-Pyrroline-5-Carboxylate Dehydrogenase
Biochemistry
Organ Specificity
Mitochondrial matrix
NAD+ kinase
Tumor Suppressor Protein p53
Peptides
Reactive Oxygen Species
Subjects
Details
- ISSN :
- 0166445X
- Volume :
- 208
- Database :
- OpenAIRE
- Journal :
- Aquatic Toxicology
- Accession number :
- edsair.doi.dedup.....87f86878dfef9fafbf82615e37db7060