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Pressure-induced changes on the morphology and gene expression in mammalian cells
- Source :
- Biology Open, Vol 10, Iss 7 (2021), Biology Open, article-version (VoR) Version of Record
- Publication Year :
- 2021
- Publisher :
- The Company of Biologists, 2021.
-
Abstract
- We evaluated the effect of high hydrostatic pressure on mouse embryonic fibroblasts (MEFs) and mouse embryonic stem (ES) cells. Hydrostatic pressures of 15, 30, 60, and 90 MPa were applied for 10 min, and changes in gene expression were evaluated. Among genes related to mechanical stimuli, death-associated protein 3 was upregulated in MEF subjected to 90 MPa pressure; however, other genes known to be upregulated by mechanical stimuli did not change significantly. Genes related to cell differentiation did not show a large change in expression. On the other hand, genes related to pluripotency, such as Oct4 and Sox2, showed a twofold increase in expression upon application of 60 MPa hydrostatic pressure for 10 min. Although these changes did not persist after overnight culture, cells that were pressurized to 15 MPa showed an increase in pluripotency genes after overnight culture. When mouse ES cells were pressurized, they also showed an increase in the expression of pluripotency genes. These results show that hydrostatic pressure activates pluripotency genes in mammalian cells. This article has an associated First Person interview with the first author of the paper.<br />Summary: Application of high hydrostatic pressure on somatic cells induce changes in gene expression including upregulation in pluripotency genes.
- Subjects :
- 0301 basic medicine
QH301-705.5
Cellular differentiation
Science
Hydrostatic pressure
Biophysics
Gene Expression
Biology
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Mechanobiology
Mice
0302 clinical medicine
SOX2
Downregulation and upregulation
Gene expression
Hydrostatic Pressure
Animals
Biology (General)
Gene
SOXB1 Transcription Factors
Cell Differentiation
Fibroblasts
mechanobiology
embryonic stem cells
pluripotency
Embryonic stem cell
Cell biology
mouse embryonic fibroblast
030104 developmental biology
General Agricultural and Biological Sciences
Octamer Transcription Factor-3
030217 neurology & neurosurgery
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 20466390
- Volume :
- 10
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Biology Open
- Accession number :
- edsair.doi.dedup.....88093180a9af22ebf85654e6360b9e1b