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Interactions of daidzin with intramolecular G-quadruplex
- Source :
- FEBS letters. 580(20)
- Publication Year :
- 2006
-
Abstract
- The potential interaction of daidzin, an ingredient of soy isoflavones, with human telomeric antiparallel G-quadruplex dAG(3)(T(2)AG(3))(3) was studied using ESI-MS, PAGE, CD and molecular simulation. Experimental studies indicated that daidzin molecules interacted with dAG(3)(T(2)AG(3))(3) and formed DNA-daidzin complex with the stoichiometric ratio of 1:1 and 1:2. The transition temperature of the G-quadruplex increased at higher ratio of daidzin to DNA. Under molecular crowding conditions the interactions between daidzin and the G-quadruplex become much stronger. Combining computational simulation and experimental results, it is demonstrated that the dAG(3)(T(2)AG(3))(3)/daidzin complex with a stoichiometric ratio of 1:1 is stabilized through the pi-pi conjugacy interactions and hydrogen bondings between daidzin and the bases of G-quadruplex. This work provides guidance not only on exploring the molecular anti-cancer mechanism of dietary isoflavones, but also searching small natural products as promising anticancer candidates that can inhibit telomerase activity.
- Subjects :
- Models, Molecular
Guanine
Stereochemistry
Macromolecular Substances
Biophysics
Daidzin
G-quadruplex
Antiparallel (biochemistry)
Biochemistry
chemistry.chemical_compound
Structural Biology
Molecular-crowding conditions
Genetics
Molecule
Humans
Enzyme Inhibitors
Molecular Biology
Repetitive Sequences, Nucleic Acid
Molecular Structure
Circular Dichroism
Nucleic Acid Heteroduplexes
Hydrogen Bonding
Cell Biology
Isoflavones
Anticancer
chemistry
Intramolecular force
Nucleic Acid Conformation
Stoichiometry
DNA
Subjects
Details
- ISSN :
- 00145793
- Volume :
- 580
- Issue :
- 20
- Database :
- OpenAIRE
- Journal :
- FEBS letters
- Accession number :
- edsair.doi.dedup.....884402ddf989b825e2aee3a0316fd902