Back to Search
Start Over
Sequence-structure based phylogeny of GPCR Class A Rhodopsin receptors
- Source :
- Molecular phylogenetics and evolution. 74
- Publication Year :
- 2013
-
Abstract
- Current methods of G protein coupled receptors (GPCRs) phylogenetic classification are sequence based and therefore inappropriate for highly divergent sequences, sharing low sequence identity. In this study, sequence structure profile based alignment generated by PROMALS3D was used to understand the GPCR Class A Rhodopsin superfamily evolution using the MEGA 5 software. Phylogenetic analysis included a combination of Neighbor-Joining method and Maximum Likelihood method, with 1000 bootstrap replicates. Our study was able to identify potential ligand association for Class A Orphans and putative/unclassified Class A receptors with no cognate ligand information: GPR21 and GPR52 with fatty acids; GPR75 with Neuropeptide Y; GPR82, GPR18, GPR141 with N-arachidonylglycine; GPR176 with Free fatty acids, GPR10 with Tachykinin & Neuropeptide Y; GPR85 with ATP, ADP & UDP glucose; GPR151 with Galanin; GPR153 and GPR162 with Adrenalin, Noradrenalin; GPR146, GPR139, GPR142 with Neuromedin, Ghrelin, Neuromedin U-25 & Thyrotropin-releasing hormone; GPR171 with ATP, ADP & UDP Glucose; GPR88, GPR135, GPR161, GPR101with 11-cis-retinal; GPR83 with Tackykinin; GPR148 with Prostanoids, GPR109b, GPR81, GPR31with ATP & UTP and GPR150 with GnRH I & GnRHII. Furthermore, we suggest that this study would prove useful in re-classification of receptors, selecting templates for homology modeling and identifying ligands which may show cross reactivity with other GPCRs as signaling via multiple ligands play a significant role in disease modulation.
- Subjects :
- Likelihood Functions
Rhodopsin
Class C GPCR
Sequence Analysis, DNA
Biology
Neuropeptide Y receptor
Receptors, G-Protein-Coupled
Evolution, Molecular
Biochemistry
Genetics
biology.protein
GPR18
Animals
Humans
Homology modeling
Galanin
Receptor
Molecular Biology
Ecology, Evolution, Behavior and Systematics
Phylogeny
G protein-coupled receptor
Subjects
Details
- ISSN :
- 10959513
- Volume :
- 74
- Database :
- OpenAIRE
- Journal :
- Molecular phylogenetics and evolution
- Accession number :
- edsair.doi.dedup.....884958bfd113b1ec18c3b8d523a3d294