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Diabetes Induction in C57BL/6 Mice Reconstituted with Lymphocytes of Nonobese Diabetic <-> C57BL/6 Mouse Embryo Aggregation Chimeras
- Source :
- Scandinavian Journal of Immunology. 48:571-576
- Publication Year :
- 1998
- Publisher :
- Wiley, 1998.
-
Abstract
- To determine whether the genetic background of the insulin-producing beta cells of the pancreas contributes to autoimmune diabetes susceptibility, we have used a model of the disease based on transferring spleen cells from nonobese diabetic (NOD) C57BL/6 (B6) embryo aggregation (EA) chimeras into B6 and NOD irradiated mice. Insulitis and diabetes could be induced into both B6 and NOD hosts, albeit with low incidence. Cyclophosphamide (CY) treatment, known to accelerate diabetes in prediabetic NOD mice, was found to increase diabetes incidence up to 50-60% in both B6 and NOD mice reconstituted with chimeric splenocytes, while diabetes did not occur in CY-treated B6 mice reconstituted with B6 splenocytes. We conclude that the genetic make-up of the target organ does not affect the final stage of the pathogenesis of insulin-dependent diabetes mellitus.
- Subjects :
- Male
medicine.medical_specialty
Adoptive cell transfer
Immunology
Spleen
Nod
Biology
Mice
Mice, Inbred NOD
Diabetes mellitus
Internal medicine
medicine
Splenocyte
Animals
Lymphocytes
Cyclophosphamide
NOD mice
Transplantation Chimera
General Medicine
medicine.disease
Adoptive Transfer
Mice, Inbred C57BL
Diabetes Mellitus, Type 1
Endocrinology
medicine.anatomical_structure
Female
Insulitis
Biobreeding rat
Subjects
Details
- ISSN :
- 13653083 and 03009475
- Volume :
- 48
- Database :
- OpenAIRE
- Journal :
- Scandinavian Journal of Immunology
- Accession number :
- edsair.doi.dedup.....886b9846a6bbc0498113d19a504e03ef