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The bZIP Transcription Factor Fgap1 Mediates Oxidative Stress Response and Trichothecene Biosynthesis But Not Virulence in Fusarium graminearum
- Source :
- PLoS ONE, PLoS ONE, Vol 8, Iss 12, p e83377 (2013), Plos One 12 (8), 12 p.. (2013), PLoS ONE, Public Library of Science, 2013, 8 (12), 12 p. ⟨10.1371/journal.pone.0083377⟩
- Publication Year :
- 2013
- Publisher :
- Public Library of Science, 2013.
-
Abstract
- International audience; Redox sensing is of primary importance for fungi to cope with oxidant compounds found in their environment. Plant pathogens are particularly subject to the oxidative burst during the primary steps of infection. In the budding yeast Saccharomyces cerevisiae, it is the transcription factor Yap1 that mediates the response to oxidative stress via activation of genes coding for detoxification enzymes. In the cereal pathogen Fusarium graminearum, Fgap1 a homologue of Yap1 was identified and its role was investigated. During infection, this pathogen produces mycotoxins belonging to the trichothecenes family that accumulate in the grains. The global regulation of toxin biosynthesis is not completely understood. However, it is now clearly established that an oxidative stress activates the production of toxins by F. graminearum. The involvement of Fgap1 in this activation was investigated. A deleted mutant and a strain expressing a truncated constitutive form of Fgap1 were constructed. None of the mutants was affected in pathogenicity. The deleted mutant showed higher level of trichothecenes production associated with overexpression of Tri genes. Moreover activation of toxin accumulation in response to oxidative stress was no longer observed. Regarding the mutant with the truncated constitutive form of Fgap1, toxin production was strongly reduced. Expression of oxidative stress response genes was not activated in the deleted mutant and expression of the gene encoding the mitochondrial superoxide dismutase MnSOD1 was up-regulated in the mutant with the truncated constitutive form of Fgap1. Our results demonstrate that Fgap1 plays a key role in the link between oxidative stress response and F. graminearum secondary metabolism.
- Subjects :
- [SDV]Life Sciences [q-bio]
Science
Saccharomyces cerevisiae
Trichothecene
Mutant
trichothecene
champignon pathogène
Biology
biosynthèse
medicine.disease_cause
Microbiology
Fungal Proteins
03 medical and health sciences
pcr
Fusarium
Gene Expression Regulation, Fungal
fusarium graminearum
Gene expression
medicine
saccharomyces cerevisiae
Transcription factor
030304 developmental biology
Regulation of gene expression
0303 health sciences
Multidisciplinary
030306 microbiology
Fungal genetics
stress oxydatif
food and beverages
biology.organism_classification
toxine
Oxidative Stress
Basic-Leucine Zipper Transcription Factors
Medicine
Trichothecenes
facteur de transcription
Oxidative stress
expression des gènes
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 8
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....886b9e20839678d665e3394d6e77e981