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Prevalence of Linda Virus Neutralizing Antibodies in the Austrian Pig Population
- Source :
- Viruses, Volume 13, Issue 6, Viruses, Vol 13, Iss 1001, p 1001 (2021)
- Publication Year :
- 2021
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2021.
-
Abstract
- A novel pestivirus species, termed Lateral-shaking Inducing Neuro-Degenerative Agent virus (LindaV), was discovered in a piglet-producing farm in Austria in 2015 related to severe congenital tremor cases. Since the initial outbreak LindaV has not been found anywhere else. In this study, we determined the seroprevalence of LindaV infections in the domestic pig population of Austria. A fluorophore labeled infectious cDNA clone of LindaV (mCherry-LindaV) was generated and used in serum virus neutralization (SVN) assays for the detection of LindaV specific neutralizing antibodies in porcine serum samples. In total, 637 sera from sows and gilts from five federal states of Austria, collected between the years 2015 and 2020, were analyzed. We identified a single serum showing a high neutralizing antibody titer, that originated from a farm (Farm S2) in the proximity of the initially affected farm. The analysis of 57 additional sera from Farm S2 revealed a wider spread of LindaV in this pig herd. Furthermore, a second LindaV strain originating from this farm could be isolated in cell culture and was further characterized at the genetic level. Possible transmission routes and virus reservoir hosts of this emerging porcine virus need to be addressed in future studies.
- Subjects :
- 0301 basic medicine
Male
040301 veterinary sciences
Swine
animal diseases
Population
Sus scrofa
serum virus neutralization assay
Antibodies, Viral
Microbiology
Virus
Article
0403 veterinary science
03 medical and health sciences
Seroepidemiologic Studies
Virology
Prevalence
Seroprevalence
Animals
education
Neutralizing antibody
Swine Diseases
novel Linda virus strain
education.field_of_study
biology
seroprevalence
Pestivirus
Pestivirus Infections
Outbreak
04 agricultural and veterinary sciences
pestiviruses
biology.organism_classification
Antibodies, Neutralizing
QR1-502
Titer
Domestic pig
030104 developmental biology
Infectious Diseases
Austria
biology.protein
Female
Linda virus
Subjects
Details
- Language :
- English
- ISSN :
- 19994915
- Database :
- OpenAIRE
- Journal :
- Viruses
- Accession number :
- edsair.doi.dedup.....886f6f6e6a250d4a2d30d1d64bbae66e
- Full Text :
- https://doi.org/10.3390/v13061001