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Erratum to 'Mold Alkaloid Cytochalasin D Modifies the Morphology and Secretion of fMLP-, LPS-, or PMA-Stimulated Neutrophils upon Adhesion to Fibronectin'
- Source :
- Mediators of Inflammation, Mediators of Inflammation, Vol 2018 (2018)
- Publication Year :
- 2018
- Publisher :
- Hindawi, 2018.
-
Abstract
- Neutrophils play an essential role in innate immunity due to their ability to migrate into infected tissues and kill microbes with bactericides located in their secretory granules. Neutrophil transmigration and degranulation are tightly regulated by actin cytoskeleton. Invading pathogens produce alkaloids that cause the depolymerization of actin, such as the mold alkaloid cytochalasin D. We studied the effect of cytochalasin D on the morphology and secretion of fMLP-, LPS-, or PMA-stimulated human neutrophils upon adhesion to fibronectin. Electron microscopy showed that the morphology of the neutrophils adherent to fibronectin in the presence of various stimuli differed. But in the presence of cytochalasin D, all stimulated neutrophils exhibited a uniform nonspread shape and developed thread-like membrane tubulovesicular extensions (cytonemes) measuring 200 nm in diameter. Simultaneous detection of neutrophil secretory products by mass spectrometry showed that all tested stimuli caused the secretion of MMP-9, a key enzyme in the neutrophil migration. Cytochalasin D impaired the MMP-9 secretion but initiated the release of cathepsin G and other granular bactericides, proinflammatory agents. The release of bactericides apparently occurs through the formation, shedding, and lysis of cytonemes. The production of alkaloids which modify neutrophil responses to stimulation via actin depolymerization may be part of the strategy of pathogen invasion.
- Subjects :
- Lipopolysaccharides
Cathepsin G
Cytochalasin D
Neutrophils
Immunology
Cell Biology
Bridged Bicyclo Compounds, Heterocyclic
Fibronectins
N-Formylmethionine Leucyl-Phenylalanine
Matrix Metalloproteinase 9
Cell Movement
lcsh:Pathology
Cell Adhesion
Humans
Tetradecanoylphorbol Acetate
Thiazolidines
Erratum
Cells, Cultured
lcsh:RB1-214
Subjects
Details
- Language :
- English
- ISSN :
- 14661861 and 09629351
- Volume :
- 2018
- Database :
- OpenAIRE
- Journal :
- Mediators of Inflammation
- Accession number :
- edsair.doi.dedup.....88810c05cba93588d13111fa37409ce5