Direct Analysis of Doping Agents in Raw Urine Using Hydrophobic Paper Spray Mass Spectrometry

Made available in DSpace on 2020-12-12T02:09:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-06-03 In this study, the direct analysis of doping agents in urine samples with no sample preparation by a modified paper spray mass spectrometry (PS-MS) methodology has been demonstrated for the first time. We have described a paper surface treatment with trichloromethylsilane using a gas-phase reaction to increase the ionization of target compounds. This approach was applied for the analysis of two classes of banned substances in urine samples: anabolic agents (trenbolone and clenbuterol) and diuretics (furosemide and hydrochlorothiazide). Under optimized conditions, the developed methods presented satisfactory repeatability, and an analysis of variance showed linearity without lack-of-fit. Highly sensitive detections as low as sub-nanogram per milliliter levels, which is below the minimum required performance levels proposed by the World Anti-Doping Agency, have been reached using the hydrophobic PS-MS analysis without any preconcentration and cleanup step. Department of Chemistry and Biochemistry Ohio State University, 100 West 18th Avenue Institute of Chemistry Department of Analytical Chemistry São Paulo State University (UNESP), Rua Professor Francisco Degni 55 ,P.O. Box 355 Institute of Chemistry Department of Analytical Chemistry São Paulo State University (UNESP), Rua Professor Francisco Degni 55 ,P.O. Box 355