Back to Search
Start Over
Tetrahydrocurcumin Ameliorates Skin Inflammation by Modulating Autophagy in High-Fat Diet-Induced Obese Mice
- Source :
- BioMed Research International, Vol 2021 (2021), BioMed Research International
- Publication Year :
- 2021
- Publisher :
- Hindawi Limited, 2021.
-
Abstract
- Obesity can induce chronic low-grade inflammation via oxidative stress. Tetrahydrocurcumin (THC) is a major curcumin metabolite with anti-inflammatory and antioxidant effects, but little is known about its effects on the skin of obese individuals. Thus, the aim of this study was to investigate the effects of THC on inflammatory cytokine production, oxidative stress, and autophagy in the skin of mice with high-fat diet- (HFD-) induced obesity. Eight-week-old C57BL/6J mice were fed a regular diet, HFD (60% of total calories from fat), or HFD supplemented with THC (100 mg/kg/day orally) for 12 weeks. We measured their body weights during the experimental period. After 12-week treatments, we performed western blotting and real-time polymerase chain reaction analyses on skin samples to evaluate the expression of inflammatory cytokines, oxidative stress markers, and autophagy markers. We observed higher tumor necrosis factor-α (TNF-α), NADPH oxidase 2 (Nox2), Nox4, and phosphorylated p65 levels; lower nuclear factor erythroid 2-related factor 2 (Nrf2) expression; and higher light chain 3 (LC3), autophagy-related 5 (Atg5), and Beclin 1 expression in the skin of HFD mice compared to the corresponding levels in the skin of mice fed with regular diet. THC administration decreased TNF-α, Nox2, Nox4, and phosphorylated p65 levels and activated the Nrf2 pathway. Interestingly, THC administration suppressed the expression of the autophagy markers LC3, Atg5, and Beclin 1. Overall, HFD-fed mice exhibited an elevation in inflammation, oxidative stress, and autophagy in their skin. THC ameliorated obesity-related skin pathology, and therefore, it is a potential therapeutic agent for obesity-related inflammatory skin diseases.
- Subjects :
- Male
0301 basic medicine
medicine.medical_treatment
Anti-Inflammatory Agents
Mice, Obese
medicine.disease_cause
Antioxidants
Mice
030207 dermatology & venereal diseases
0302 clinical medicine
Medicine
Skin
NADPH oxidase
biology
Temperature
General Medicine
Cytokine
Cytokines
Tumor necrosis factor alpha
medicine.symptom
Signal Transduction
Research Article
medicine.medical_specialty
Curcumin
Article Subject
ATG5
Inflammation
Diet, High-Fat
Skin Diseases
General Biochemistry, Genetics and Molecular Biology
Proinflammatory cytokine
03 medical and health sciences
Internal medicine
Autophagy
Animals
Obesity
General Immunology and Microbiology
business.industry
Body Weight
Mice, Inbred C57BL
Oxidative Stress
030104 developmental biology
Endocrinology
biology.protein
business
Oxidative stress
Subjects
Details
- ISSN :
- 23146141 and 23146133
- Volume :
- 2021
- Database :
- OpenAIRE
- Journal :
- BioMed Research International
- Accession number :
- edsair.doi.dedup.....88d70eeb99ba60f048890f12a70dfa49