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Dose response and time course of manganese-enhanced magnetic resonance imaging for visual pathway tracing in vivo
- Source :
- Neural Regeneration Research, Vol 11, Iss 7, Pp 1185-1190 (2016), Neural Regeneration Research
- Publication Year :
- 2016
- Publisher :
- Wolters Kluwer Medknow Publications, 2016.
-
Abstract
- Axonal tracing is useful for detecting optic nerve injury and regeneration, but many commonly used methods cannot be used to observe axoplasmic flow and synaptic transmission in vivo. Manganese (Mn(2+))-enhanced magnetic resonance imaging (MEMRI) can be used for in vivo longitudinal tracing of the visual pathway. Here, we explored the dose response and time course of an intravitreal injection of MnCl2 for tracing the visual pathway in rabbits in vivo using MEMRI. We found that 2 mM MnCl2 enhanced images of the optic nerve but not the lateral geniculate body or superior colliculus, whereas at all other doses tested (5-40 mM), images of the visual pathway from the retina to the contralateral superior colliculus were significantly enhanced. The images were brightest at 24 hours, and then decreased in brightness until the end of the experiment (7 days). No signal enhancement was observed in the visual cortex at any concentration of MnCl2. These results suggest that MEMRI is a viable method for temporospatial tracing of the visual pathway in vivo. Signal enhancement in MEMRI depends on the dose of MnCl2, and the strongest signals appear 24 hours after intravitreal injection.
- Subjects :
- genetic structures
Neurotransmission
optic nerve
lcsh:RC346-429
030218 nuclear medicine & medical imaging
nerve regeneration
manganese
03 medical and health sciences
0302 clinical medicine
Developmental Neuroscience
In vivo
medicine
magnetic resonance imaging
visual pathway
tracing
in vivo
intravitreal injection
time
dose
neural regeneration
lcsh:Neurology. Diseases of the nervous system
Retina
medicine.diagnostic_test
Chemistry
Superior colliculus
Magnetic resonance imaging
Visual cortex
medicine.anatomical_structure
Axoplasmic transport
Optic nerve
Neuroscience
030217 neurology & neurosurgery
Research Article
Biomedical engineering
Subjects
Details
- Language :
- English
- ISSN :
- 16735374
- Volume :
- 11
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Neural Regeneration Research
- Accession number :
- edsair.doi.dedup.....8909990e6838dcfc5c0229d7f944c7ed