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CRISP2 Is a Regulator of Multiple Aspects of Sperm Function and Male Fertility
- Source :
- Endocrinology. 160(4)
- Publication Year :
- 2018
-
Abstract
- The cysteine-rich secretory proteins (CRISPs) are a group of proteins that show a pronounced expression biased to the male reproductive tract. Although sperm encounter CRISPs at virtually all phases of sperm development and maturation, CRISP2 is the sole CRISP produced during spermatogenesis, wherein it is incorporated into the developing sperm head and tail. In this study we tested the necessity for CRISP2 in male fertility using Crisp2 loss-of-function mouse models. In doing so, we revealed a role for CRISP2 in establishing the ability of sperm to undergo the acrosome reaction and in establishing a normal flagellum waveform. Crisp2-deficient sperm possess a stiff midpiece and are thus unable to manifest the rapid form of progressive motility seen in wild type sperm. As a consequence, Crisp2-deficient males are subfertile. Furthermore, a yeast two-hybrid screen and immunoprecipitation studies reveal that CRISP2 can bind to the CATSPER1 subunit of the Catsper ion channel, which is necessary for normal sperm motility. Collectively, these data define CRISP2 as a determinant of male fertility and explain previous clinical associations between human CRISP2 expression and fertility.
- Subjects :
- 0301 basic medicine
Male
medicine.medical_specialty
media_common.quotation_subject
Acrosome reaction
Motility
030209 endocrinology & metabolism
Fertility
Biology
Flagellum
Male infertility
03 medical and health sciences
Mice
0302 clinical medicine
Endocrinology
Internal medicine
medicine
Animals
Spermatogenesis
Sperm motility
Infertility, Male
media_common
Mice, Knockout
urogenital system
Acrosome Reaction
Membrane Proteins
medicine.disease
Sperm
Spermatozoa
Cell biology
030104 developmental biology
Sperm Motility
Cell Adhesion Molecules
Subjects
Details
- ISSN :
- 19457170
- Volume :
- 160
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Endocrinology
- Accession number :
- edsair.doi.dedup.....8917eeb58d39f7c49dfb403aec8f69ba