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Tobacco smoke-associated N7-alkylguanine in DNA of larynx tissue and leucocytes
- Source :
- Carcinogenesis. 17:501-506
- Publication Year :
- 1996
- Publisher :
- Oxford University Press (OUP), 1996.
-
Abstract
- The presence of N7-alkylguanine adducts in DNA was analysed in a group of 46 patients with larynx tumours. All patients were subjected to laryngectomy and the tissues accessible for analysis by (32)P-post-labelling assay were larynx tumour, larynx non-tumour and peripheral blood leucocytes. N7-Alkylguanine adducts were detected in all the studied DNA samples. The average level of N7-alkylguanines was 26.2/10(7) nucleotides in tumour cells, 22.7/10(7) in non-tumour cells and 13.1/10(7) in blood leucocytes. There was significantly higher level of N7-alkylguanines in the larynx tissues in males than in females. The effect of tobacco smoking on DNA adduct levels was shown by an increase in the average levels of N7-alkylguanines in the subject groups classified according to their smoking habits. A moderate age-related increase in levels of N7-alkylguanine was demonstrated in larynx tumour tissue. The levels of N7-alkylguanine adducts in larynx cells were compared with that of aromatic DNA adducts. Pearson correlation coefficients (0.28 for tumour tissue and 0.30 for non-tumour tissue) indicate independent formation and removal of N7-alkylguanine and aromatic DNA adducts resulting from tobacco smoke exposure.
- Subjects :
- Adult
Male
Larynx
Alkylating Agents
Cancer Research
Pathology
medicine.medical_specialty
Guanine
medicine.medical_treatment
Tobacco smoke
DNA Adducts
chemistry.chemical_compound
Smoke
Tobacco
DNA adduct
Leukocytes
medicine
Humans
Nucleotide
Laryngeal Neoplasms
Aged
chemistry.chemical_classification
General Medicine
Middle Aged
Laryngeal Neoplasm
In vitro
Laryngectomy
Plants, Toxic
medicine.anatomical_structure
chemistry
Female
DNA
Subjects
Details
- ISSN :
- 14602180 and 01433334
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- Carcinogenesis
- Accession number :
- edsair.doi.dedup.....893ae400369b259e4f0a89ee7844fd52