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Molecular and genetic characterization of barley mutants and genetic mapping of mutant rpr2 required for Rpg1-mediated resistance against stem rust
Molecular and genetic characterization of barley mutants and genetic mapping of mutant rpr2 required for Rpg1-mediated resistance against stem rust
- Source :
- Theoretical and Applied Genetics. 129:1519-1529
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- This study describes the generation, screening, genetic and molecular characterization, and high-resolution mapping of barley mutants susceptible to stem rust ( Puccinia graminis f. sp. tritici ) races MCCF and HKHJ. A single gene, Rpg1, has protected barley cultivars against many races of stem rust pathogen (Puccinia graminis f. sp. tritici) for the last 70 years in the United States and Canada. To identify signaling components of protein product RPG1, we employed a mutagenesis approach. Using this approach, six mutants exhibiting susceptibility to Puccinia graminis f. sp. tritici races MCCF and HKHJ were identified in the gamma irradiated M2 population of resistant cultivar Morex, which carries Rpg1 on chromosome 7H. The mutants retained a functional Rpg1 gene and an apparently functional protein, suggesting that the mutated genes were required for downstream or upstream signaling. Selected mutants were non-allelic, hence each mutant represents a unique gene. Low and high-resolution genetic mapping of the rpr2 mutant identified chromosome 6H (bin 6) as the location of the mutated gene. The target region was reduced to 0.6 cM and gene content analyzed. Based on the published barley genomic sequence, the target region contains approximately 157 genes, including a set that encodes putative leucine-rich receptor-like protein kinases, which may be strong candidates for the gene of interest. Overall, this study presents a strong platform for future map-based cloning of genes identified in this mutant screen.
- Subjects :
- 0106 biological sciences
0301 basic medicine
DNA, Plant
Mutant
Population
Mutagenesis (molecular biology technique)
Genes, Plant
Stem rust
01 natural sciences
Chromosomes, Plant
03 medical and health sciences
Gene mapping
Genetics
education
Gene
Disease Resistance
Plant Diseases
Puccinia
Cloning
education.field_of_study
biology
Basidiomycota
food and beverages
Hordeum
General Medicine
Physical Chromosome Mapping
biology.organism_classification
Phenotype
030104 developmental biology
Gamma Rays
Mutation
Agronomy and Crop Science
010606 plant biology & botany
Biotechnology
Subjects
Details
- ISSN :
- 14322242 and 00405752
- Volume :
- 129
- Database :
- OpenAIRE
- Journal :
- Theoretical and Applied Genetics
- Accession number :
- edsair.doi.dedup.....894295c0a09bd2428cbb7734a115ae68
- Full Text :
- https://doi.org/10.1007/s00122-016-2721-3