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SNAIL Transctiption factor in prostate cancer cells promotes neurite outgrowth
- Source :
- Biochimie
- Publication Year :
- 2020
-
Abstract
- Neurite outgrowth involves reciprocal signaling interactions between tumor cells and nerves where invading tumor cells have acquired the ability to respond to pro-invasive signals within the nerve environment. Neurite outgrowth could serve as a mechanism leading to invasion of cancer cells into the nerve sheath and subsequent metastasis. Snail transcription factor can promote migration and invasion of prostate cancer cells. We hypothesized that prostate cancer cell interaction with nerve cells will be mediated by Snail expression within prostate cancer cells. For this study we utilized various prostate cancer cell lines: C4-2 non-silencing (NS, control); C4-2 Snail shRNA, (stable Snail knockdown); LNCaP Neo (empty vector control) and LNCaP Snail (stably over-expressing Snail). Cancer cell adhesion and migration towards nerve cells (snF96.2 or NS20Y) was examined by co-culture assays. Conditioned media (CM) collected from C4-2 cells was cultured with nerve cells (PC-12 or NS20Y) for 48 h followed by qualitative or quantitative neurite outgrowth assay. Our results showed that cancer cells expressing high levels of Snail (LNCaP Snail/C4-2 NS) displayed significantly higher migration adherence to nerve cells, compared to cells with lower levels of Snail (LNCaP Neo/C4-2 Snail shRNA). Additionally, LNCaP Snail or C4-2 NS (Snail-high) CM led to a higher neurite outgrowth compared to the LNCaP Neo or C4-2 Snail shRNA (Snail-low). In conclusion, Snail promotes migration and adhesion to nerve cells, as well as neurite outgrowth via secretion of soluble factors. Therefore, targeting cancer cell interaction with nerves may contribute to halting prostate cancer progression/metastasis.
- Subjects :
- 0301 basic medicine
Male
Neurite
Neuronal Outgrowth
Snail
Cell Communication
Biochemistry
Article
Metastasis
Cell Line
Small hairpin RNA
03 medical and health sciences
Prostate cancer
Mice
Cell Movement
biology.animal
LNCaP
parasitic diseases
medicine
Cell Adhesion
Animals
Humans
Gene Silencing
Cell Proliferation
Neurons
Gene knockdown
030102 biochemistry & molecular biology
biology
fungi
Prostatic Neoplasms
General Medicine
medicine.disease
Rats
030104 developmental biology
Cancer cell
Cancer research
Snail Family Transcription Factors
Subjects
Details
- ISSN :
- 16386183
- Volume :
- 180
- Database :
- OpenAIRE
- Journal :
- Biochimie
- Accession number :
- edsair.doi.dedup.....8945b683e0a9091d30bfa25d0298f150