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Identification of Panton-Valentine leukocidin virulence gene in methicillin—resistant Staphylococcus aureus isolated from clinical specimens of burn patients in Zare Hospitals of Sari, Iran
- Source :
- Journal of Acute Disease, Vol 9, Iss 3, Pp 121-125 (2020)
- Publication Year :
- 2020
- Publisher :
- Wolters Kluwer Medknow Publications, 2020.
-
Abstract
- Objective: To identify the pathogenic gene of Panton-Valentine leukocidin in methicillin-resistant Staphylococcus aureus strain isolated from clinical specimens of burn patients in Shaheed Zare Burn Hospital. Methods: A total of 104 samples of Staphylococcus were collected and 78 aureus samples were isolated from Zare Hospital patients from November 2016 to July 2017. All isolates were identified using a standard biochemical and laboratory methodology in accordance with CLSI principles, and disk agar diffusion antibiogram were performed to identify methicillin resistant colonies. Then the presence of the Panton-Valentine leukocidin gene was tested by PCR. Results: Of the methicillin-resistant Staphylococcus aureus samples, 80% were negative for the Panton-Valentine leukocidin gene, and only 20% of the samples had Panton-Valentine leukocidin gene. Male and female patients showed no significant difference in the positivity rate of Panton-Valentine leukocidin gene (16.12% vs. 33.33%) (P=0.25). Besides, there was no significant difference in bacterial resistance or susceptibility to antibiotics between samples with or without the Panton-Valentine leukocidin gene. Conclusions: In recent years, increased resistance has been a serious threat. The resistance or susceptibility of Staphylococcus aureus strains to different antibiotics is different in different geographical locations.
- Subjects :
- 050402 sociology
medicine.drug_class
Antibiotics
Leukocidin
Virulence
medicine.disease_cause
Microbiology
03 medical and health sciences
0302 clinical medicine
Antibiotic resistance
0504 sociology
medicine
methicillin
resistance
panton-valentine leukocidin
pcr
staphylococcus aureus
skin and connective tissue diseases
business.industry
05 social sciences
lcsh:Medical emergencies. Critical care. Intensive care. First aid
General Medicine
lcsh:RC86-88.9
respiratory system
biochemical phenomena, metabolism, and nutrition
bacterial infections and mycoses
Methicillin-resistant Staphylococcus aureus
body regions
Staphylococcus aureus
Panton–Valentine leukocidin
business
Staphylococcus
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 25895516 and 22216189
- Volume :
- 9
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of Acute Disease
- Accession number :
- edsair.doi.dedup.....89556e54cb90c282b237f07e5cc9dccd