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Identification of a splice variant of mouse TRPA1 that regulates TRPA1 activity
Identification of a splice variant of mouse TRPA1 that regulates TRPA1 activity
- Source :
- Nature Communications
- Publication Year :
- 2013
- Publisher :
- Springer Science and Business Media LLC, 2013.
-
Abstract
- Transient receptor potential ankyrin 1 (TRPA1) protein is a nonselective cation channel. Although many studies suggest that TRPA1 is involved in inflammatory and neuropathic pain, its mechanism remains unclear. Here we identify an alternative splice variant of the mouse Trpa1 gene. TRPA1a (full-length) and TRPA1b (splice variant) physically interact with each other and TRPA1b increases the expression of TRPA1a in the plasma membrane. TRPA1a and TRPA1b co-expression significantly increases current density in response to different agonists without affecting their single-channel conductance. Exogenous overexpression of Trpa1b gene in wild-type and TRPA1KO DRG neurons also increases TRPA1a-mediated AITC responses. Moreover, expression levels of Trpa1a and Trpa1b mRNAs change dynamically in two pain models (complete Freund's adjuvant-induced inflammatory pain and partial sciatic nerve ligation-induced neuropathic pain models). These results suggest that TRPA1 may be regulated through alternative splicing under these pathological conditions.
- Subjects :
- Boron Compounds
Molecular Sequence Data
General Physics and Astronomy
Bioinformatics
General Biochemistry, Genetics and Molecular Biology
Mice
Transient receptor potential channel
Transient Receptor Potential Channels
Isothiocyanates
Ganglia, Spinal
Animals
Humans
Ankyrin
Amino Acid Sequence
RNA, Messenger
Cloning, Molecular
TRPA1 Cation Channel
Gene
chemistry.chemical_classification
Multidisciplinary
Behavior, Animal
Chemistry
Cell Membrane
Alternative splicing
HEK 293 cells
Correction
General Chemistry
Immunohistochemistry
Rats
Cell biology
Alternative Splicing
HEK293 Cells
Models, Animal
Neuropathic pain
Sciatic nerve
Capsaicin
Ion Channel Gating
Protein Binding
Subjects
Details
- ISSN :
- 20411723
- Volume :
- 4
- Database :
- OpenAIRE
- Journal :
- Nature Communications
- Accession number :
- edsair.doi.dedup.....895582f85b2ffe2b964c1afdaae2d360
- Full Text :
- https://doi.org/10.1038/ncomms3399