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Genotoxicity potential of 8-Cl-cyclic adenosine monophosphate assessed with cytogenetic tests in vivo
- Source :
- Archives of Medical Research
- Publication Year :
- 2004
- Publisher :
- Elsevier Science Inc, New York, 2004.
-
Abstract
- Background Growth-modulating noncytotoxic activity of 8-chloro-adenosine 3′,5′-cyclic monophosphate (8-Cl-cAMP) showed inhibitory effect on growth of a wide variety of cancer cell lines in vitro and in vivo . To assess possible genotoxic effects of 8-Cl-cAMP, we conducted a study in vivo using male BALB/c mice. Methods Clastogenic effects were estimated by bone marrow micronucleus assay and cytogenetic test in adult mice BALB/c strain. 8-Cl-cAMP was administered intraperitoneally (i.p.) to three dose groups including 10 mg/kg body weight (b.w.), 90 mg/kg b.w., and 160 mg/kg b.w., with saline solution as negative control and cyclophosphamide, a known mutagen, and clastogen as positive control during a 7-day period in 24-h intervals. Results Micronucleus test in vivo results showed consistently increasing dose-dependent pattern increase of dose regime (10 mg/kg body weight [b.w.], 90 mg/kg b.w., and 160 mg/kg b.w.), and increase in frequency of micronuclei in polychromatic erythrocytes (4.88 ± 0.35, 8.32 ± 0.57, and 11.74 ± 0.37) compared to negative control (2.04 ± 0.28). Quantitative effects are paralleled by structural changes in chromosome morphology. 8-Cl-cAMP induced structural (breaks, gaps, centric rings, acentrics, and Robertsonian translocations) and numerical-type chromosomal aberrations (aneuploidy and polyploidy). Conclusions Results of this study demonstrate that 8-Cl-cAMP has genotoxic potential in vivo .
- Subjects :
- antitumor agent 8-Cl-cAMP
Erythrocytes
8-Bromo Cyclic Adenosine Monophosphate
Chromosomal translocation
Mutagen
Antineoplastic Agents
Pharmacology
Biology
medicine.disease_cause
Chromosomes
03 medical and health sciences
Clastogen
chemistry.chemical_compound
Mice
0302 clinical medicine
In vivo
Cell Line, Tumor
medicine
Animals
Cyclic adenosine monophosphate
Cyclophosphamide
030304 developmental biology
Chromosome Aberrations
0303 health sciences
Mice, Inbred BALB C
Micronucleus Tests
numerical and structural chromosomal aberrations
Body Weight
genotoxicity
General Medicine
In vitro
3. Good health
micronucleus test
chemistry
030220 oncology & carcinogenesis
Immunology
Micronucleus test
Genotoxicity
DNA Damage
Mutagens
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Archives of Medical Research
- Accession number :
- edsair.doi.dedup.....895b1b41b035bbe0add1090f249d92a2