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PHLPP2 is regulated by competing endogenous RNA network in pathogenesis of colon cancer
- Source :
- Aging (Albany NY)
- Publication Year :
- 2020
- Publisher :
- Impact Journals, LLC, 2020.
-
Abstract
- Recently, homologous pleckstrin-homology (PH)-domain leucine-rich-repeat protein phosphatases (PHLPP2) has been reported as a tumor suppressor in colon cancer. This study aimed to unravel the possible involvement of long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) regulating PHLPP2 in colon cancer. Expressions of candidate lncRNAs and miRNAs were verified by the RT-qPCR and Western blot analyses in colon cancer. The roles of candidate genes in colon cancer were investigated in HT-29 cells in vitro and in mouse tumor xenograft model in vivo. PHLPP2, a target of miR-141 and miR-424, was downregulated in colon cancer. PHLPP2 upregulation and miR-141 and miR-424 downregulation suppressed the colon cancer cell proliferation, migration, invasion, and epithelial-mesenchymal transition, and promote cell apoptosis, which also resulted in suppression of tumor metastasis and formation. Furthermore, LINC00402, LINC00461, and SFTA1P were identified as the targets of miR-141 and miR-424 and acted as competitive endogenous RNAs (ceRNAs) of PHLPP2. The upregulation of LINC00402, LINC00461, and SFTA1P was verified to enhance the suppressive effects of PHLPP2 in the pathogenesis of colon cancer. Conjointly, our results demonstrated the suppressive effects of PHLPP2 in colon cancer and proved that LINC00402, LINC00461, and SFTA1P acted as ceRNAs of PHLPP2 by competitive binding to miR-141 and miR-424.
- Subjects :
- Adult
Male
Aging
Colorectal cancer
Mice, Nude
Biology
Metastasis
Mice
PHLPP2
lncRNA
Downregulation and upregulation
Western blot
In vivo
microRNA
Phosphoprotein Phosphatases
medicine
Animals
Humans
Neoplasm Metastasis
miRNA
Aged
Cell Proliferation
Aged, 80 and over
medicine.diagnostic_test
Competing endogenous RNA
EMT
ceRNA
Cell Biology
Middle Aged
medicine.disease
MicroRNAs
Apoptosis
Colonic Neoplasms
Cancer research
Heterografts
Female
RNA, Long Noncoding
HT29 Cells
Research Paper
Subjects
Details
- ISSN :
- 19454589
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Aging
- Accession number :
- edsair.doi.dedup.....896d4b990964410d3f9979f34dee873f