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Identification of a Novel Nuclear Localization Signal Sequence in Chlamydia trachomatis-Secreted Hypothetical Protein CT311
- Source :
- PLoS ONE, PLoS ONE, Vol 8, Iss 5, p e64529 (2013)
- Publication Year :
- 2013
- Publisher :
- Public Library of Science, 2013.
-
Abstract
- We previously reported that Chlamydia trachomatis hypothetical protein CT311 was secreted out of chlamydial inclusion and into host cell cytosol. We now found that CT311 further entered host cell nucleus at the late stage of infection and continued to accumulate in the nucleus of C. trachomatis-infected cells. When CT311 was expressed via a transgene in mammalian cells, CT311 protein was exclusively detected in the nucleus, suggesting that CT311 by itself is sufficient for nuclear targeting. However, preexisting nuclear CT311 did not affect subsequent chlamydial infection. Using deletion constructs, we mapped a nuclear localization signal sequence of CT311 to residues 21 to 63 ((21)AVEGKPLSRAAQLRERRKDLHVSGKPSPRYALKKRALEAKKNK(63)). This sequence was sufficient for targeting a heterologous protein into mammalian cell nucleus and it contains two independent clusters of basic residues ((34)RERRK(38) and (53)KKRALEAKKNK(63) respectively). Deletion or alanine substitution of the basic residues in either cluster led to loss of nuclear targeting activity, suggesting that both clusters are critical for the nuclear targeting function. These observations have demonstrated that the hypothetical protein CT311 possesses a novel nuclear localization signal sequence with dual modules of basic residues for targeting host cell nucleus during Chlamydia trachomatis infection.
- Subjects :
- Nuclear Localization Signals
lcsh:Medicine
Chlamydia trachomatis
medicine.disease_cause
Biochemistry
Molecular Cell Biology
Gram Negative
Chlamydia
lcsh:Science
Peptide sequence
Host cell nucleus
0303 health sciences
Multidisciplinary
Host cell cytosol
Obstetrics and Gynecology
Cellular Structures
3. Good health
Cell biology
Bacterial Pathogens
medicine.anatomical_structure
Infectious Diseases
Host-Pathogen Interactions
Medicine
Research Article
Urology
Hypothetical protein
Molecular Sequence Data
Sexually Transmitted Diseases
Active Transport, Cell Nucleus
Biology
Microbiology
Peptide Mapping
03 medical and health sciences
Bacterial Proteins
medicine
Humans
Amino Acid Sequence
030304 developmental biology
Cell Nucleus
030306 microbiology
Genitourinary Infections
lcsh:R
Proteins
Virology
Cytosol
Cell nucleus
lcsh:Q
Nuclear localization sequence
HeLa Cells
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 8
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....8976874f8a6e6dbb0c4ba1237905ae97