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Functional analysis of seven regulators of G protein signaling (RGSs) in the nematode-trapping fungus Arthrobotrys oligospora
- Source :
- Virulence, Vol 12, Iss 1, Pp 1825-1840 (2021), Virulence, article-version (VoR) Version of Record
- Publication Year :
- 2021
- Publisher :
- Taylor & Francis Group, 2021.
-
Abstract
- Regulators of G protein signaling (RGSs) are proteins that negatively regulate G protein signal transduction. In this study, seven putative RGSs were characterized in the nematode-trapping (NT) fungus, Arthrobotrys oligospora. Deleting Rgs genes significantly increased intracellular cAMP levels, and caused defects in mycelia growth, stress resistance, conidiation, trap formation, and nematocidal activity. In particular, the ΔAoFlbA mutant was unable to produce conidia and traps. Transcriptomic analysis showed that amino acid metabolic and biosynthetic processes were significantly enriched in the ΔAoFlbA mutant compared to WT. Interestingly, Gas1 family genes are significantly expanded in A. oligospora and other NT fungi that produce adhesive traps, and are differentially expressed during trap formation in A. oligospora. Disruption of two Gas1 genes resulted in defective conidiation, trap formation, and pathogenicity. Our results indicate that RGSs play pleiotropic roles in regulating A. oligospora mycelial growth, development, and pathogenicity. Further, AoFlbA is a prominent member and required for conidiation and trap formation, possibly by regulating amino acid metabolism and biosynthesis. Our results provide a basis for elucidating the signaling mechanism of vegetative growth, lifestyle transition, and pathogenicity in NT fungi.
- Subjects :
- Microbiology (medical)
Arthrobotrys oligospora
transcriptional analysis
Nematoda
G protein
Immunology
arthrobotrys oligospora
Conidiation
Fungus
Infectious and parasitic diseases
RC109-216
conidiation
Microbiology
Fungal Proteins
regulator of g protein signaling (rgs)
Ascomycota
GTP-Binding Proteins
Animals
Transcriptional analysis
Amino Acids
biology
Functional analysis
biology.organism_classification
Cell biology
Infectious Diseases
Nematode
trap formation
Parasitology
Signal transduction
gas1 family genes
Research Article
Research Paper
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 21505608 and 21505594
- Volume :
- 12
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Virulence
- Accession number :
- edsair.doi.dedup.....89e24d3fff6d0f99303a23ace9c72081